Hangover links nuclear RNA signaling to cAMP regulation via the phosphodiesterase 4d ortholog dunce
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-171950
- The hangover gene defines a cellular stress pathway that is required for rapid ethanol tolerance in Drosophila melanogaster. To understand how cellular stress changes neuronal function, we analyzed Hangover function on a cellular and neuronal level. We provide evidence that Hangover acts as a nuclear RNA binding protein and we identified the phosphodiesterase 4d ortholog dunce as a target RNA. We generated a transcript-specific dunce mutant that is impaired not only in ethanol tolerance but also in the cellular stress response. At the neuronalThe hangover gene defines a cellular stress pathway that is required for rapid ethanol tolerance in Drosophila melanogaster. To understand how cellular stress changes neuronal function, we analyzed Hangover function on a cellular and neuronal level. We provide evidence that Hangover acts as a nuclear RNA binding protein and we identified the phosphodiesterase 4d ortholog dunce as a target RNA. We generated a transcript-specific dunce mutant that is impaired not only in ethanol tolerance but also in the cellular stress response. At the neuronal level, Dunce and Hangover are required in the same neuron pair to regulate experience-dependent motor output. Within these neurons, two cyclic AMP (cAMP)-dependent mechanisms balance the degree of tolerance. The balance is achieved by feedback regulation of Hangover and dunce transcript levels. This study provides insight into how nuclear Hangover/RNA signaling is linked to the cytoplasmic regulation of cAMP levels and results in neuronal adaptation and behavioral changes.…
Autor(en): | Manuela Ruppert, Mirjam Franz, Anastasios Saratis, Laura Velo Escarcena, Oliver Hendrich, Li Ming Gooi, Isabell Schwenkert, Ansgar Klebes, Henrike Scholz |
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URN: | urn:nbn:de:bvb:20-opus-171950 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Cell Reports |
Erscheinungsjahr: | 2017 |
Band / Jahrgang: | 18 |
Heft / Ausgabe: | 2 |
Seitenangabe: | 533-544 |
Originalveröffentlichung / Quelle: | Cell Reports (2017) 18:2, pp. 533-544. https://doi.org/10.1016/j.celrep.2016.12.048 |
DOI: | https://doi.org/10.1016/j.celrep.2016.12.048 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/28076795 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Freie Schlagwort(e): | Drosophila melanogaster; Dunce isoforms; PDE4d; alcohol tolerance; biology; cellular stress; dunce; hangover |
Datum der Freischaltung: | 11.02.2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |