Complexation with C\(_{60}\) fullerene increases doxorubicin efficiency against leukemic cells in vitro
Please always quote using this URN: urn:nbn:de:bvb:20-opus-228257
- Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C\(_{60}\) fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.Here, we studied the physicochemical properties and anticancer activity of C\(_{60}\) fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C\(_{60}\)-Doxorubicin complexes in a ratio 1:1 and 2:1Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C\(_{60}\) fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells.Here, we studied the physicochemical properties and anticancer activity of C\(_{60}\) fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C\(_{60}\)-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug's level evidenced C\(_{60}\) fullerene considerable nanocarrier function.The results of this study indicated that C\(_{60}\) fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.…
Author: | Anna Grebinyk, Svitlana Prylutska, Sergii Grebinyk, Yuriy Prylutskyy, Uwe Ritter, Olga Matyshevska, Thomas Dandekar, Marcus Frohme |
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URN: | urn:nbn:de:bvb:20-opus-228257 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Nanoscale Research Letters |
Year of Completion: | 2019 |
Volume: | 14 |
Issue: | 61 |
Source: | Nanoscale Research Letters (2019) 14:61. https://doi.org/10.1186/s11671-019-2894-1 |
DOI: | https://doi.org/10.1186/s11671-019-2894-1 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | C-60 fullerene; accumulation; cytotoxicity; doxorubicin; leukemic cells; noncovalent complex |
Release Date: | 2023/01/27 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |