Supramolecular fluorescence resonance energy transfer in nucleobase-modified fluorogenic RNA aptamers
Please always quote using this URN: urn:nbn:de:bvb:20-opus-203084
- RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Here we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to bound ligands DMHBI+ or DMHBO+. The photophysical properties of the new nucleobase-ligand-FRET pair revealed structural restraints for the overall RNA aptamer organizationRNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Here we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to bound ligands DMHBI+ or DMHBO+. The photophysical properties of the new nucleobase-ligand-FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET-based readout of ligand binding. This strategy is generally suitable for binding site mapping and may also be applied for responsive aptamer devices.…
Author: | Christian Steinmetzger, Carmen Bäuerlein, Claudia HöbartnerORCiD |
---|---|
URN: | urn:nbn:de:bvb:20-opus-203084 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Language: | English |
Parent Title (English): | Angewandte Chemie, International Edition |
Year of Completion: | 2020 |
Volume: | 59 |
Pagenumber: | 6760–6764 |
Source: | Angewandte Chemie, International Edition 2020, 59, 6760–6764. DOI:10.1002/anie.201916707 |
DOI: | https://doi.org/10.1002/anie.201916707 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 547 Organische Chemie |
Tag: | Fluoreszenzresonanz-Energietransfer; Isomorphe Nukleobasen-Analoga; RNA-Aptamere; Stokes-Verschiebung; Struktursonden RNA aptamers; fluorescence resonance energy transfer; isomorphic nucleobase analog; large stokes shift; stokes shift; structure probes; structure probing |
Release Date: | 2020/04/23 |
EU-Project number / Contract (GA) number: | 682586 |
OpenAIRE: | OpenAIRE |
Note: | Parallel erschienen in Angewandte Chemie 2020,132, 6826–6830. DOI: 10.1002/ange.201916707 (Deutsche Ausgabe). |
Licence (German): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |