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Adult Raphe-Specific Deletion of Lmx1b Leads to Central Serotonin Deficiency
Please always quote using this URN: urn:nbn:de:bvb:20-opus-133581
- The transcription factor Lmx1b is essential for the differentiation and survival of central serotonergic (5-HTergic) neurons during embryonic development. However, the role of Lmx1b in adult 5-HTergic neurons is unknown. We used an inducible Cre-LoxP system to selectively inactivate Lmx1b expression in the raphe nuclei of adult mice. Pet1-CreER(T2) mice were generated and crossed with Lmx1b(flox/flox) mice to obtain Pet1-CreER(T2); Lmx1b(flox/flox) mice (which termed as Lmx1b iCKO). After administration of tamoxifen, the level of 5-HT in theThe transcription factor Lmx1b is essential for the differentiation and survival of central serotonergic (5-HTergic) neurons during embryonic development. However, the role of Lmx1b in adult 5-HTergic neurons is unknown. We used an inducible Cre-LoxP system to selectively inactivate Lmx1b expression in the raphe nuclei of adult mice. Pet1-CreER(T2) mice were generated and crossed with Lmx1b(flox/flox) mice to obtain Pet1-CreER(T2); Lmx1b(flox/flox) mice (which termed as Lmx1b iCKO). After administration of tamoxifen, the level of 5-HT in the brain of Lmx1b iCKO mice was reduced to 60% of that in control mice, and the expression of tryptophan hydroxylase 2 (Tph2), serotonin transporter (Sert) and vesicular monoamine transporter 2 (Vmat2) was greatly down-regulated. On the other hand, the expression of dopamine and norepinephrine as well as aromatic L-amino acid decarboxylase (Aadc) and Pet1 was unchanged. Our results reveal that Lmx1b is required for the biosynthesis of 5-HT in adult mouse brain, and it may be involved in maintaining normal functions of central 5-HTergic neurons by regulating the expression of Tph2, Sert and Vmat2.…
Author: | Ning-Ning Song, Jian-Bo Xiu, Ying Huang, Jia-Yin Chen, Lei Zhang, Lise Gutknecht, Klaus Peter Lesch, He Li, Yu-Qiang Ding |
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URN: | urn:nbn:de:bvb:20-opus-133581 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Klinik und Poliklinik für Psychiatrie, Psychosomatik und Psychotherapie |
Language: | English |
Parent Title (English): | PLoS ONE |
Year of Completion: | 2011 |
Volume: | 6 |
Issue: | 1 |
Pagenumber: | e15998 |
Source: | PLoS ONE 6(1): e15998. doi:10.1371/journal.pone.0015998 |
DOI: | https://doi.org/10.1371/journal.pone.0015998 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | Brain; Disorders; Expression; Mice; Molecular-genetics; Neurons; PET-1; Sonic hedgehog; System; Transporter |
Release Date: | 2019/03/06 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |