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Functional Neuronal Cells Generated by Human Parthenogenetic Stem Cells

Please always quote using this URN: urn:nbn:de:bvb:20-opus-130268
  • Parent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. We determined the potential of hpESCs to differentiate into various neural subtypes. Concurrently, we examined DNA methylation and expression status of imprinted genes. Under culture conditions promoting neural differentiation, hpESC-derived neural stem cells (hpNSCs) gave rise toParent of origin imprints on the genome have been implicated in the regulation of neural cell type differentiation. The ability of human parthenogenetic (PG) embryonic stem cells (hpESCs) to undergo neural lineage and cell type-specific differentiation is undefined. We determined the potential of hpESCs to differentiate into various neural subtypes. Concurrently, we examined DNA methylation and expression status of imprinted genes. Under culture conditions promoting neural differentiation, hpESC-derived neural stem cells (hpNSCs) gave rise to glia and neuron-like cells that expressed subtype-specific markers and generated action potentials. Analysis of imprinting in hpESCs and in hpNSCs revealed that maternal-specific gene expression patterns and imprinting marks were generally maintained in PG cells upon differentiation. Our results demonstrate that despite the lack of a paternal genome, hpESCs generate proliferating NSCs that are capable of differentiation into physiologically functional neuron-like cells and maintain allele-specific expression of imprinted genes. Thus, hpESCs can serve as a model to study the role of maternal and paternal genomes in neural development and to better understand imprinting-associated brain diseases.show moreshow less

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Metadaten
Author: Ruhel Ahmad, Wanja Wolber, Sigrid Eckardt, Philipp Koch, Jessica Schmitt, Ruslan Semechkin, Christian Geis, Manfred Heckmann, Oliver Brüstle, John K. McLaughlin, Anna-Leena Sirén, Albrecht M. Müller
URN:urn:nbn:de:bvb:20-opus-130268
Document Type:Journal article
Faculties:Medizinische Fakultät / Neurochirurgische Klinik und Poliklinik
Medizinische Fakultät / Institut für Medizinische Strahlenkunde und Zellforschung
Language:English
Parent Title (English):PLoS One
Year of Completion:2012
Volume:7
Issue:8
Pagenumber:e42800
Source:PLoS ONE 7(8): e42800. doi:10.1371/journal.pone.0042800
DOI:https://doi.org/10.1371/journal.pone.0042800
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:blastocysts; brain development; derivation; differentiation; in-vitro; lines; methylation; mice; pluripotent; specification
Release Date:2016/11/28
EU-Project number / Contract (GA) number:222943
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung