- Treffer 1 von 1
Tom70 Is Essential for PINK1 Import into Mitochondria
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-131061
- PTEN induced kinase 1 (PINK1) is a serine/threonine kinase in the outer membrane of mitochondria (OMM), and known as a responsible gene of Parkinson's disease (PD). The precursor of PINK1 is synthesized in the cytosol and then imported into the mitochondria via the translocase of the OMM (TOM) complex. However, a large part of PINK1 import mechanism remains unclear. In this study, we examined using cell-free system the mechanism by which PINK1 is targeted to and assembled into mitochondria. Surprisingly, the main component of the importPTEN induced kinase 1 (PINK1) is a serine/threonine kinase in the outer membrane of mitochondria (OMM), and known as a responsible gene of Parkinson's disease (PD). The precursor of PINK1 is synthesized in the cytosol and then imported into the mitochondria via the translocase of the OMM (TOM) complex. However, a large part of PINK1 import mechanism remains unclear. In this study, we examined using cell-free system the mechanism by which PINK1 is targeted to and assembled into mitochondria. Surprisingly, the main component of the import channel, Tom40 was not necessary for PINK1 import. Furthermore, we revealed that the import receptor Tom70 is essential for PINK1 import. In addition, we observed that although PINK1 has predicted mitochondrial targeting signal, it was not processed by the mitochondrial processing peptidase. Thus, our results suggest that PINK1 is imported into mitochondria by a unique pathway that is independent of the TOM core complex but crucially depends on the import receptor Tom70.…
Autor(en): | Hiroki Kato, Qiping Lu, Doron Rapaport, Vera Kozjak-Pavlovic |
---|---|
URN: | urn:nbn:de:bvb:20-opus-131061 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS ONE |
Erscheinungsjahr: | 2013 |
Band / Jahrgang: | 8 |
Heft / Ausgabe: | 3 |
Seitenangabe: | e58435 |
Originalveröffentlichung / Quelle: | PLoS ONE 8(3): e58435. doi:10.1371/ journal.pone.0058435 |
DOI: | https://doi.org/10.1371/journal.pone.0058435 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 611 Menschliche Anatomie, Zytologie, Histologie |
Freie Schlagwort(e): | Parkinsons diesease; binding; biogenesis; complex; intracellular membranes; mechanisms; outer-membrane proteins; pathway; quality control; recruitment |
Datum der Freischaltung: | 12.05.2016 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |