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Isolation of a functional human interleukin 2 gene from a cosmid library by recombination in vivo
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-62662
- A method has been developed that allows the isolation of genomic clones from a cosmid library by homologaus recombination in vivo. This method was used to isolate a human genomic interleukin 2 (IL2) gene. The genomic cosmid library was packaged in vivo into A. phage particles. A recombination-proficient host strain carrying IL2 cDNA sequences in a non-homologaus plasmid vector was infected by the packaged cosmid library. After in vivo packaging and reinfection, recombinants carrying the antibiotic resistance genes of both vectors were selected.A method has been developed that allows the isolation of genomic clones from a cosmid library by homologaus recombination in vivo. This method was used to isolate a human genomic interleukin 2 (IL2) gene. The genomic cosmid library was packaged in vivo into A. phage particles. A recombination-proficient host strain carrying IL2 cDNA sequences in a non-homologaus plasmid vector was infected by the packaged cosmid library. After in vivo packaging and reinfection, recombinants carrying the antibiotic resistance genes of both vectors were selected. From a recombinant cosmid clone the chromosomal IL2 genewas restored. After DNA mediated gene transfer into mouse Ltk- cells human IL2 was expressed constitutively.…
Autor(en): | W. Lindenmaier, K. E. Dittmar, H. Hauser, A. Necker, Walter Sebald |
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URN: | urn:nbn:de:bvb:20-opus-62662 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Erscheinungsjahr: | 1985 |
Originalveröffentlichung / Quelle: | In: Gene (1985) 39(1) 33-39. |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Normierte Schlagworte (GND): | Biochemie |
Freie Schlagwort(e): | DNA mediated gene transfer; Recombinant DNA; bacteriophage lambda; expression plasmid; packaging; screening |
Datum der Freischaltung: | 26.04.2012 |
Lizenz (Deutsch): | Deutsches Urheberrecht |