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Super-resolution microscopy of the synaptic active zone

Please always quote using this URN: urn:nbn:de:bvb:20-opus-148997
  • Brain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and evenBrain function relies on accurate information transfer at chemical synapses. At the presynaptic active zone (AZ) a variety of specialized proteins are assembled to complex architectures, which set the basis for speed, precision and plasticity of synaptic transmission. Calcium channels are pivotal for the initiation of excitation-secretion coupling and, correspondingly, capture a central position at the AZ. Combining quantitative functional studies with modeling approaches has provided predictions of channel properties, numbers and even positions on the nanometer scale. However, elucidating the nanoscopic organization of the surrounding protein network requires direct ultrastructural access. Without this information, knowledge of molecular synaptic structure-function relationships remains incomplete. Recently, super-resolution microscopy (SRM) techniques have begun to enter the neurosciences. These approaches combine high spatial resolution with the molecular specificity of fluorescence microscopy. Here, we discuss how SRM can be used to obtain information on the organization of AZ proteinsshow moreshow less

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Metadaten
Author: Nadine Ehmann, Markus Sauer, Robert J. Kittel
URN:urn:nbn:de:bvb:20-opus-148997
Document Type:Journal article
Faculties:Medizinische Fakultät / Physiologisches Institut
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Frontiers in Cellular Neuroscience
Year of Completion:2015
Volume:9
Issue:7
Source:Frontiers in Cellular Neuroscience 9:7 (2015). DOI: 10.3389/fncel.2015.00007
DOI:https://doi.org/10.3389/fncel.2015.00007
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:Ca\(^{2+}\) channels; active zone; excitation-secretion coupling; neurotransmitter release; presynaptic calcium; structure-function relationships; super-resolution microscopy
Release Date:2018/11/23
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International