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Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes

Please always quote using this URN: urn:nbn:de:bvb:20-opus-32087
  • Semi -iso la ted annul a te lamellae were prepared from single newt oocy tes (Triturus alpestris) by a modified Call a n-T omlin technique. Such preparations were examined with the electron mi croscope, and the negative sta ining a ppearance of th e a nnulate lamellae is described . The annul a te lamellae can be de tected either adhering to the nuclear envelope or being detached from it. Sometimes they a re obse rved to be connected with slender tubular-like structures interpreted as pa rts of the endoplasmic reti culum. The results obta inedSemi -iso la ted annul a te lamellae were prepared from single newt oocy tes (Triturus alpestris) by a modified Call a n-T omlin technique. Such preparations were examined with the electron mi croscope, and the negative sta ining a ppearance of th e a nnulate lamellae is described . The annul a te lamellae can be de tected either adhering to the nuclear envelope or being detached from it. Sometimes they a re obse rved to be connected with slender tubular-like structures interpreted as pa rts of the endoplasmic reti culum. The results obta ined from negativ e sta ining a re combined with those from sections. Especially, the structural data on th e a nnula te lamellae and the nuclear envelope of the very same cell were compa red . Evidence is presented th a t in the oocytes studied the two kinds of porous cisternae, n amely a nnul a te lamellae and nuclear envelope, a re markedly distinguished in that the annul a te lamellae ex hibit a much higher pore frequency (generally about twice tha t found for the corresponding nuclear envelope) and have al so a rela tive pore area occupying as much as 32 % to 55 % of th e cistern al surface (compa red with 13 % to 22 % in the nuclear envelopes). T he pore di ame ter a nd all other ultras tructural details of the pore complexes, however, a re equi valent in both kinds of porous cisternae. Like the annuli of the nuclear pore complexes of various a nimal and pl ant cells, the a nnuli of the a nnula te lamellae pores reveal al so an eightfold symmetry of their subunits in negatively stained as well as in ectioned ma teria l. Furthermore, th e a nnul a te lamellae a re shown to be a site of activity of the Mg-Na-Kstimul a ted ATPase.show moreshow less

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Metadaten
Author: Ulrich Scheer, Werner W. Franke
URN:urn:nbn:de:bvb:20-opus-32087
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Year of Completion:1969
Source:In: Journal of Cell Biology (1969) 42, 2, 519-533.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Release Date:2009/09/25