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Reovirus-specific messenger ribonucleoprotein particles from Hela cells

Please always quote using this URN: urn:nbn:de:bvb:20-opus-47028
  • When reovirus-infected Hela cells are incubated at 43°C virus-specific messenger RNA is released ~rom the polysomes. It accumulates free in the cytoplasm as messenger ribonucleoprotem partIcles (mRNPs). The:e part~cles have a sedimentati~n rate of about 50S and a buoyant densIty m CsCI of 1.42 g/cm . ReovIrus mRNPs contam, beSIdes all three size classes of reovirus messenger RNA, the same spectrum of proteins found in the polysomal mRNPs from uninfected cells, plus t~o addi~ional pr?teins with molecular masses of 7000~ d and 110000 d,When reovirus-infected Hela cells are incubated at 43°C virus-specific messenger RNA is released ~rom the polysomes. It accumulates free in the cytoplasm as messenger ribonucleoprotem partIcles (mRNPs). The:e part~cles have a sedimentati~n rate of about 50S and a buoyant densIty m CsCI of 1.42 g/cm . ReovIrus mRNPs contam, beSIdes all three size classes of reovirus messenger RNA, the same spectrum of proteins found in the polysomal mRNPs from uninfected cells, plus t~o addi~ional pr?teins with molecular masses of 7000~ d and 110000 d, respectively. Electron mIcroscoPIc exammatlOn of the reovIrus mRNP fractIOn reveals specific Y-shaped structures wIth a total mean length ofO.5Ilm.show moreshow less

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Metadaten
Author: Jürgen Kreft
URN:urn:nbn:de:bvb:20-opus-47028
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Year of Completion:1980
Source:In: Zeitschrift für Naturforschung (1980) 35, C, 1046-1051
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Electron Microscopy; Hela Cells; Messenger Ribonucleoprotein Particles; Reovirus; mRNP-Proteins
Release Date:2010/07/13
Licence (German):License LogoDeutsches Urheberrecht