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Mms1 binds to G-rich regions in Saccharomyces cerevisiae and influences replication and genome stability

Please always quote using this URN: urn:nbn:de:bvb:20-opus-170577
  • The regulation of replication is essential to preserve genome integrity. Mms1 is part of the E3 ubiquitin ligase complex that is linked to replication fork progression. By identifying Mms1 binding sites genome-wide in Saccharomyces cerevisiae we connected Mms1 function to genome integrity and replication fork progression at particular G-rich motifs. This motif can form G-quadruplex (G4) structures in vitro. G4 are stable DNA structures that are known to impede replication fork progression. In the absence of Mms1, genome stability is at risk atThe regulation of replication is essential to preserve genome integrity. Mms1 is part of the E3 ubiquitin ligase complex that is linked to replication fork progression. By identifying Mms1 binding sites genome-wide in Saccharomyces cerevisiae we connected Mms1 function to genome integrity and replication fork progression at particular G-rich motifs. This motif can form G-quadruplex (G4) structures in vitro. G4 are stable DNA structures that are known to impede replication fork progression. In the absence of Mms1, genome stability is at risk at these G-rich/G4 regions as demonstrated by gross chromosomal rearrangement assays. Mms1 binds throughout the cell cycle to these G-rich/G4 regions and supports the binding of Pif1 DNA helicase. Based on these data we propose a mechanistic model in which Mms1 binds to specific G-rich/G4 motif located on the lagging strand template for DNA replication and supports Pif1 function, DNA replication and genome integrity.show moreshow less

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Metadaten
Author: Katharina Wanzek, Eike Schwindt, John A. Capra, Katrin Paeschke
URN:urn:nbn:de:bvb:20-opus-170577
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Nucleic Acids Research
Year of Completion:2017
Volume:45
Issue:13
Pagenumber:7796-7806
Source:Nucleic Acids Research, 2017, Vol. 45, No. 13, 7796-7806. DOI: 10.1093/nar/gkx467
DOI:https://doi.org/10.1093/nar/gkx467
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28535251
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie
Tag:Saccharomyces cerevisiae; genome integrity; regulation; replication
Release Date:2019/09/27
EU-Project number / Contract (GA) number:638988
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International