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Proteomic Analysis of Excretory-Secretory Products of Mesocestoides corti Metacestodes Reveals Potential Suppressors of Dendritic Cell Functions

Please always quote using this URN: urn:nbn:de:bvb:20-opus-166742
  • Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metacestode larva (tetrathyridium) of the tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES products but not larval homogenates inhibit the stimuli-driven release of the pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs). Within the ES fraction,Accumulating evidences have assigned a central role to parasite-derived proteins in immunomodulation. Here, we report on the proteomic identification and characterization of immunomodulatory excretory-secretory (ES) products from the metacestode larva (tetrathyridium) of the tapeworm Mesocestoides corti (syn. M. vogae). We demonstrate that ES products but not larval homogenates inhibit the stimuli-driven release of the pro-inflammatory, Th1-inducing cytokine IL-12p70 by murine bone marrow-derived dendritic cells (BMDCs). Within the ES fraction, we biochemically narrowed down the immunosuppressive activity to glycoproteins since active components were lipid-free, but sensitive to heat- and carbohydrate-treatment. Finally, using bioassay-guided chromatographic analyses assisted by comparative proteomics of active and inactive fractions of the ES products, we defined a comprehensive list of candidate proteins released by M. corti tetrathyridia as potential suppressors of DC functions. Our study provides a comprehensive library of somatic and ES products and highlight some candidate parasite factors that might drive the subversion of DC functions to facilitate the persistence of M. corti tetrathyridia in their hosts.show moreshow less

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Metadaten
Author: Emilia Vendelova, Jeferson Camargo de Lima, Karina Rodrigues Lorenzatto, Karina Mariante Monteiro, Thomas Mueller, Jyotishman Veepaschit, Clemens Grimm, Klaus Brehm, Gabriela Hrčková, Manfred B. Lutz, Henrique B. Ferreira, Justin Komguep Nono
URN:urn:nbn:de:bvb:20-opus-166742
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Hygiene und Mikrobiologie
Medizinische Fakultät / Institut für Virologie und Immunbiologie
Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):PLoS Neglected Tropical Diseases
Year of Completion:2016
Volume:10
Issue:10
Pagenumber:e0005061
Source:PLoS Neglected Tropical Diseases 10(10):e0005061 (2016). DOI: 10.1371/journal.pntd.0005061
DOI:https://doi.org/10.1371/journal.pntd.0005061
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Mesocestoides corti; excretory-secretory; proteomic analysis
Release Date:2019/07/10
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International