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Genetic Dissection of Aversive Associative Olfactory Learning and Memory in Drosophila Larvae

Please always quote using this URN: urn:nbn:de:bvb:20-opus-166672
  • Memory formation is a highly complex and dynamic process. It consists of different phases, which depend on various neuronal and molecular mechanisms. In adult Drosophila it was shown that memory formation after aversive Pavlovian conditioning includes—besides other forms—a labile short-term component that consolidates within hours to a longer-lasting memory. Accordingly, memory formation requires the timely controlled action of different neuronal circuits, neurotransmitters, neuromodulators and molecules that were initially identified byMemory formation is a highly complex and dynamic process. It consists of different phases, which depend on various neuronal and molecular mechanisms. In adult Drosophila it was shown that memory formation after aversive Pavlovian conditioning includes—besides other forms—a labile short-term component that consolidates within hours to a longer-lasting memory. Accordingly, memory formation requires the timely controlled action of different neuronal circuits, neurotransmitters, neuromodulators and molecules that were initially identified by classical forward genetic approaches. Compared to adult Drosophila, memory formation was only sporadically analyzed at its larval stage. Here we deconstruct the larval mnemonic organization after aversive olfactory conditioning. We show that after odor-high salt conditioning larvae form two parallel memory phases; a short lasting component that depends on cyclic adenosine 3’5’-monophosphate (cAMP) signaling and synapsin gene function. In addition, we show for the first time for Drosophila larvae an anesthesia resistant component, which relies on radish and bruchpilot gene function, protein kinase C activity, requires presynaptic output of mushroom body Kenyon cells and dopamine function. Given the numerical simplicity of the larval nervous system this work offers a unique prospect for studying memory formation of defined specifications, at full-brain scope with single-cell, and single-synapse resolution.show moreshow less

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Metadaten
Author: Annekathrin Widmann, Marc Artinger, Lukas Biesinger, Kathrin Boepple, Christina Peters, Jana Schlechter, Mareike Selcho, Andreas S. Thum
URN:urn:nbn:de:bvb:20-opus-166672
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):PLoS Genetics
Year of Completion:2016
Volume:12
Issue:10
Pagenumber:e1006378
Source:PLoS Genetics 12(10):e1006378 (2016). DOI: 10.1371/journal.pgen.1006378
DOI:https://doi.org/10.1371/journal.pgen.1006378
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Drosophila; genetic dissection; memory formation
Release Date:2019/07/10
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International