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Nucleotide sequence analysis of a cloned DNA fragment from human cells reveals homology to retrotransposons

Please always quote using this URN: urn:nbn:de:bvb:20-opus-61525
  • During molecular cloning of proviral DNA of human. spumaretroVirus, various recombinant clones were estabUshed and analyzed. Blot hybridization revealed that one of the recoinbinant plasmids bad the characteristic features of a member of the long interspersed repetitive sequences famlly. The DNA element was analyzed by restrictioil mapping and nuelootide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when conipared with the 5'-terminal part of the pol gelie product of the murine retrotransposon LIMd. The 3' regionDuring molecular cloning of proviral DNA of human. spumaretroVirus, various recombinant clones were estabUshed and analyzed. Blot hybridization revealed that one of the recoinbinant plasmids bad the characteristic features of a member of the long interspersed repetitive sequences famlly. The DNA element was analyzed by restrictioil mapping and nuelootide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when conipared with the 5'-terminal part of the pol gelie product of the murine retrotransposon LIMd. The 3' region of the cloned DNA element encodes proteins witb an even higher degree of homology of 67.4% in comparison to the corresponding parts of a member of the primate Kpnl sequence family.show moreshow less

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Metadaten
Author: Rolf M. Flügel, Bernd Maurer, Helmut Bannert, Axel Rethwilm, Paul Schnitzler, Gholamreza Darai
URN:urn:nbn:de:bvb:20-opus-61525
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Virologie und Immunbiologie
Language:English
Year of Completion:1987
Source:In: MOLECULAR AND CELLULAR BIOLOGY (1987) 7, 1, 231-236
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
GND Keyword:Virologie
Release Date:2011/09/17
Licence (German):License LogoDeutsches Urheberrecht