A transcriptome derived female-specific marker from the invasive Western mosquitofish (Gambusia affinis)
Please always quote using this URN: urn:nbn:de:bvb:20-opus-144004
- Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highlySex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3' UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sex-specific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.…
Author: | Dunja K. Lamatsch, Sofia Adolfsson, Alistair M. Senior, Guntram Christiansen, Maria Pichler, Yuichi Ozaki, Linnea Smeds, Manfred Schartl, Shinichi Nakagawa |
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URN: | urn:nbn:de:bvb:20-opus-144004 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | PLoS ONE |
Year of Completion: | 2015 |
Volume: | 10 |
Issue: | 2 |
Pagenumber: | e0118214 |
Source: | PLoS ONE 10(2): e0118214 (2015). DOI: 10.1371/journal.pone.0118214 |
DOI: | https://doi.org/10.1371/journal.pone.0118214 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen |
Tag: | Cynoglossus semilaevis; Y chromosome; determination locus; determining genes; heterogamety; linkage map; poeciliid fishes; sequence alignment; sex chromosome evolution |
Release Date: | 2018/06/01 |
EU-Project number / Contract (GA) number: | 253511 |
OpenAIRE: | OpenAIRE |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |