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Chemically Induced Photoswitching of Fluorescent Probes - A General Concept for Super-Resolution Microscopy

Please always quote using this URN: urn:nbn:de:bvb:20-opus-134080
  • We review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under variousWe review fluorescent probes that can be photoswitched or photoactivated and are suited for single-molecule localization based super-resolution microscopy. We exploit the underlying photochemical mechanisms that allow photoswitching of many synthetic organic fluorophores in the presence of reducing agents, and study the impact of these on the photoswitching properties of various photoactivatable or photoconvertible fluorescent proteins. We have identified mEos2 as a fluorescent protein that exhibits reversible photoswitching under various imaging buffer conditions and present strategies to characterize reversible photoswitching. Finally, we discuss opportunities to combine fluorescent proteins with organic fluorophores for dual-color photoswitching microscopy.show moreshow less

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Metadaten
Author: Ulrike Endesfelder, Sebastian Malkusch, Benjamin Flottmann, Justine Mondry, Piotr Liguzinski, Peter J. Verveer, Mike Heilemann
URN:urn:nbn:de:bvb:20-opus-134080
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Molecules
Year of Completion:2011
Volume:16
Issue:4
Pagenumber:3106-3118
DOI:https://doi.org/10.3390/molecules16043106
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Fusion proteins; Limit; Molecules; PALM; Patterns; Photoactivated localization microscopy; Switch; Time; dSTORM; fluorescent proteins; photoswitchable organic fluorophores; super-resolution
Release Date:2019/03/18
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung