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Fluorescence localization microscopy

Please always quote using this URN: urn:nbn:de:bvb:20-opus-124416
  • Localization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the fullLocalization microscopy techniques are super-resolution fluorescence imaging methods based on the detection of individual molecules. Despite the relative simplicity of the microscope setups and the availability of commercial instruments, localization microscopy faces unique challenges. While achieving super-resolution is now routine, issues concerning data analysis and interpretation mean that revealing novel biological insights is not. Here, we outline why data analysis and the design of robust test samples may hold the key to harness the full potential of localization microscopy.show moreshow less

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Metadaten
Author: Dylan M. Owen, Markus Sauer, Katharina Gaus
URN:urn:nbn:de:bvb:20-opus-124416
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Communicative & Integrative Biology
Year of Completion:2012
Volume:5
Issue:4
Pagenumber:345-349
Source:Communicative & Integrative Biology 5:4, 345–349. doi:10.4161/cib.20348
DOI:https://doi.org/10.4161/cib.20348
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Release Date:2016/01/19
Licence (German):License LogoCC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell