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A transcriptome derived female-specific marker from the invasive Western mosquitofish (Gambusia affinis)

Please always quote using this URN: urn:nbn:de:bvb:20-opus-144004
  • Sex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highlySex-specific markers are a prerequisite for understanding reproductive biology, genetic factors involved in sex differences, mechanisms of sex determination, and ultimately the evolution of sex chromosomes. The Western mosquitofish, Gambusia affinis, may be considered a model species for sex-chromosome evolution, as it displays female heterogamety (ZW/ZZ), and is also ecologically interesting as a worldwide invasive species. Here, de novo RNA-sequencing on the gonads of sexually mature G. affinis was used to identify contigs that were highly transcribed in females but not in males (i.e., transcripts with ovary-specific expression). Subsequently, 129 primer pairs spanning 79 contigs were tested by PCR to identify sex-specific transcripts. Of those primer pairs, one female-specific DNA marker was identified, Sanger sequenced and subsequently validated in 115 fish. Sequence analyses revealed a high similarity between the identified sex-specific marker and the 3' UTR of the aminomethyl transferase (amt) gene of the closely related platyfish (Xiphophorus maculatus). This is the first time that RNA-seq has been used to successfully characterize a sex-specific marker in a fish species in the absence of a genome map. Additionally, the identified sex-specific marker represents one of only a handful of such markers in fishes.show moreshow less

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Metadaten
Author: Dunja K. Lamatsch, Sofia Adolfsson, Alistair M. Senior, Guntram Christiansen, Maria Pichler, Yuichi Ozaki, Linnea Smeds, Manfred Schartl, Shinichi Nakagawa
URN:urn:nbn:de:bvb:20-opus-144004
Document Type:Journal article
Faculties:Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):PLoS ONE
Year of Completion:2015
Volume:10
Issue:2
Pagenumber:e0118214
Source:PLoS ONE 10(2): e0118214 (2015). DOI: 10.1371/journal.pone.0118214
DOI:https://doi.org/10.1371/journal.pone.0118214
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen
Tag:Cynoglossus semilaevis; Y chromosome; determination locus; determining genes; heterogamety; linkage map; poeciliid fishes; sequence alignment; sex chromosome evolution
Release Date:2018/06/01
EU-Project number / Contract (GA) number:253511
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International