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Establishment of a New Murine Elastase-Induced Aneurysm Model Combined with Transplantation

Please always quote using this URN: urn:nbn:de:bvb:20-opus-115774
  • Introduction: The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation. Methods: Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting. Results: Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared toIntroduction: The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation. Methods: Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting. Results: Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11 +/- 0.10 mm vs. 0.75 +/- 0.03 mm; p <= 0.001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts. Conclusions: We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.show moreshow less

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Metadaten
Author: Zuzanna Rowinska, Simone Gorressen, Marc W. Merx, Thomas A. Koeppel, Elisa A. Liehn, Alma Zernecke
URN:urn:nbn:de:bvb:20-opus-115774
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Klinische Biochemie und Pathobiochemie
Language:English
Parent Title (English):PLOS ONE
ISSN:1932-6203
Year of Completion:2014
Volume:9
Issue:7
Pagenumber:e102648
Source:PLoS ONE 9(7): e102648. doi:10.1371/journal.pone.0102648
DOI:https://doi.org/10.1371/journal.pone.0102648
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=25068788
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:abdominal aortic-aneurysm; dilation; mouse models; prediction; rupture
Release Date:2015/07/21
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung