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Quantitative proteomics uncovers novel factors involved in developmental differentiation of Trypanosoma brucei

Please always quote using this URN: urn:nbn:de:bvb:20-opus-146362
  • Developmental differentiation is a universal biological process that allows cells to adapt to different environments to perform specific functions. African trypanosomes progress through a tightly regulated life cycle in order to survive in different host environments when they shuttle between an insect vector and a vertebrate host. Transcriptomics has been useful to gain insight into RNA changes during stage transitions; however, RNA levels are only a moderate proxy for protein abundance in trypanosomes. We quantified 4270 protein groups duringDevelopmental differentiation is a universal biological process that allows cells to adapt to different environments to perform specific functions. African trypanosomes progress through a tightly regulated life cycle in order to survive in different host environments when they shuttle between an insect vector and a vertebrate host. Transcriptomics has been useful to gain insight into RNA changes during stage transitions; however, RNA levels are only a moderate proxy for protein abundance in trypanosomes. We quantified 4270 protein groups during stage differentiation from the mammalian-infective to the insect form and provide classification for their expression profiles during development. Our label-free quantitative proteomics study revealed previously unknown components of the differentiation machinery that are involved in essential biological processes such as signaling, posttranslational protein modifications, trafficking and nuclear transport. Furthermore, guided by our proteomic survey, we identified the cause of the previously observed differentiation impairment in the histone methyltransferase DOT1B knock-out strain as it is required for accurate karyokinesis in the first cell division during differentiation. This epigenetic regulator is likely involved in essential chromatin restructuring during developmental differentiation, which might also be important for differentiation in higher eukaryotic cells. Our proteome dataset will serve as a resource for detailed investigations of cell differentiation to shed more light on the molecular mechanisms of this process in trypanosomes and other eukaryotes.show moreshow less

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Metadaten
Author: Mario Dejung, Ines Subota, Ferdinand Bucerius, Gülcin Dindar, Anja Freiwald, Markus Engstler, Michael Boshart, Falk Butter, Chistian J. Janzen
URN:urn:nbn:de:bvb:20-opus-146362
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):PLoS Pathogens
Year of Completion:2016
Volume:12
Issue:2
Pagenumber:e1005439
Source:PLoS Pathogens 12(2): e1005439. doi:10.1371/ journal.ppat.1005439
DOI:https://doi.org/10.1371/journal.ppat.1005439
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:cell cycle and cell division; cell differentiation; chromatin; host-pathogen interactions; parasitic cell cycles; parasitic life cycles; proteomes; transcriptome analysis
Release Date:2017/03/31
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2016
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung