• search hit 3 of 13
Back to Result List

A novel terminal-repeat retrotransposon in miniature (TRIM) is massively expressed in Echinococcus multilocularis stem cells

Please always quote using this URN: urn:nbn:de:bvb:20-opus-148306
  • Taeniid cestodes (including the human parasites Echinococcus spp. and Taenia solium) have very few mobile genetic elements (MGEs) in their genome, despite lacking a canonical PIWI pathway. The MGEs of these parasites are virtually unexplored, and nothing is known about their expression and silencing. In this work, we report the discovery of a novel family of small nonautonomous long terminal repeat retrotransposons (also known as terminal-repeat retrotransposons in miniature, TRIMs) which we have named ta-TRIM (taeniid TRIM). ta-TRIMs are onlyTaeniid cestodes (including the human parasites Echinococcus spp. and Taenia solium) have very few mobile genetic elements (MGEs) in their genome, despite lacking a canonical PIWI pathway. The MGEs of these parasites are virtually unexplored, and nothing is known about their expression and silencing. In this work, we report the discovery of a novel family of small nonautonomous long terminal repeat retrotransposons (also known as terminal-repeat retrotransposons in miniature, TRIMs) which we have named ta-TRIM (taeniid TRIM). ta-TRIMs are only the second family of TRIM elements discovered in animals, and are likely the result of convergent reductive evolution in different taxonomic groups. These elements originated at the base of the taeniid tree and have expanded during taeniid diversification, including after the divergence of closely related species such as Echinococcus multilocularis and Echinococcus granulosus. They are massively expressed in larval stages, from a small proportion of full-length copies and from isolated terminal repeats that show transcriptional read-through into downstream regions, generating novel noncoding RNAs and transcriptional fusions to coding genes. In E. multilocularis, ta-TRIMs are specifically expressed in the germinative cells (the somatic stem cells) during asexual reproduction of metacestode larvae. This would provide a developmental mechanism for insertion of ta-TRIMs into cells that will eventually generate the adult germ line. Future studies of active and inactive ta-TRIM elements could give the first clues on MGE silencing mechanisms in cestodes.show moreshow less

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author: Uriel Koziol, Santiago Radio, Pablo Smircich, Magdalena Zarowiecki, Cecilia Fernández, Klaus Brehm
URN:urn:nbn:de:bvb:20-opus-148306
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Hygiene und Mikrobiologie
Language:English
Parent Title (English):Genome Biology and Evolution
Year of Completion:2015
Volume:7
Issue:8
Pagenumber:2136-2153
Source:Genome Biology and Evolution 7(8):2136-2153 (2015). DOI: 10.1093/gbe/evv126
DOI:https://doi.org/10.1093/gbe/evv126
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:Cestoda Taeniidae; LTR retrotransposons; Schistosoma mansoni; blood fluke; epidermal growth factor; gene conversion; homologous recombination; long noncoding RNA; molecular characterization; neoblast; nonautonomous; pluripotency; retrotransposition; transposable elements
Release Date:2018/11/13
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International