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Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients

Please always quote using this URN: urn:nbn:de:bvb:20-opus-68594
  • Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain’s evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34Bacteria lose or gain genetic material and through selection, new variants become fixed in the population. Here we provide the first, genome-wide example of a single bacterial strain’s evolution in different deliberately colonized patients and the surprising insight that hosts appear to personalize their microflora. By first obtaining the complete genome sequence of the prototype asymptomatic bacteriuria strain E. coli 83972 and then resequencing its descendants after therapeutic bladder colonization of different patients, we identified 34 mutations, which affected metabolic and virulence-related genes. Further transcriptome and proteome analysis proved that these genome changes altered bacterial gene expression resulting in unique adaptation patterns in each patient. Our results provide evidence that, in addition to stochastic events, adaptive bacterial evolution is driven by individual host environments. Ongoing loss of gene function supports the hypothesis that evolution towards commensalism rather than virulence is favored during asymptomatic bladder colonization.show moreshow less

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Metadaten
Author: Jaroslaw Zdziarski, Elzbieta Brzuszkiewicz, Bjorn Wullt, Heiko Liesegang, Dvora Biran, Birgit Voigt, Jenny Gronberg-Hernandez, Bryndis Ragnarsdottir, Michael Hecker, Eliora Z. Ron, Rolf Daniel, Gerhard Gottschalk, Joerg Hacker, Catharina Svanborg, Ulrich Dobrindt
URN:urn:nbn:de:bvb:20-opus-68594
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Molekulare Infektionsbiologie
Language:English
Year of Completion:2010
Source:PLOS PATHOGENS (2010) 6, 8, DOI: 10.1371/journal.ppat.1001078
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
GND Keyword:Proteomanalyse; Bakterien
Release Date:2012/03/30
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung