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Different evolutionary modifications as a guide to rewire two-component systems

Please always quote using this URN: urn:nbn:de:bvb:20-opus-123647
  • Two-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of TCS in different organisms, this study gives detailed data, concrete alignments, and structure analysis on three general modification scenarios, whereTwo-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of TCS in different organisms, this study gives detailed data, concrete alignments, and structure analysis on three general modification scenarios, where TCS were rewired for new responses and functions: (i) exchanges in the sequence within single TCS domains, (ii) exchange of whole TCS domains; (iii) addition of new components modulating TCS function. As a result, the replacement of stimulus and promotor cassettes to rewire TCS is well defined exploiting the alignments given here. The diverged TCS examples are non-trivial and the design is challenging. Designed connector proteins may also be useful to modify TCS in selected cases.show moreshow less

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Metadaten
Author: Beate Krueger, Torben Friedrich, Frank Förster, Jörg Bernhardt, Roy Gross, Thomas Dandekar
URN:urn:nbn:de:bvb:20-opus-123647
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):Bioinformatics and Biology Insights
Year of Completion:2012
Volume:6
Pagenumber:97-128
Source:Bioinformatics and Biology Insights 2012:6 97–128. doi: 10.4137/BBI.S9356
DOI:https://doi.org/10.4137/BBI.S9356
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=PMC3348925
Dewey Decimal Classification:0 Informatik, Informationswissenschaft, allgemeine Werke / 00 Informatik, Wissen, Systeme / 004 Datenverarbeitung; Informatik
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Mycoplasma; connector; engineering; histidine kinase; promoter; response regulator; sensor; sequence alignment; synthetic biology
Release Date:2015/12/22
Note:
This is an open access article. Unrestricted non-commercial use is permitted provided the original work is properly cited.