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Integrative "Omics"-Approach Discovers Dynamic and Regulatory Features of Bacterial Stress Responses

Please always quote using this URN: urn:nbn:de:bvb:20-opus-127587
  • Bacteria constantly face stress conditions and therefore mount specific responses to ensure adaptation and survival. Stress responses were believed to be predominantly regulated at the transcriptional level. In the phototrophic bacterium Rhodobacter sphaeroides the response to singlet oxygen is initiated by alternative sigma factors. Further adaptive mechanisms include post-transcriptional and post-translational events, which have to be considered to gain a deeper understanding of how sophisticated regulation networks operate. To address thisBacteria constantly face stress conditions and therefore mount specific responses to ensure adaptation and survival. Stress responses were believed to be predominantly regulated at the transcriptional level. In the phototrophic bacterium Rhodobacter sphaeroides the response to singlet oxygen is initiated by alternative sigma factors. Further adaptive mechanisms include post-transcriptional and post-translational events, which have to be considered to gain a deeper understanding of how sophisticated regulation networks operate. To address this issue, we integrated three layers of regulation: (1) total mRNA levels at different time-points revealed dynamics of the transcriptome, (2) mRNAs in polysome fractions reported on translational regulation (translatome), and (3) SILAC-based mass spectrometry was used to quantify protein abundances (proteome). The singlet oxygen stress response exhibited highly dynamic features regarding short-term effects and late adaptation, which could in part be assigned to the sigma factors RpoE and RpoH2 generating distinct expression kinetics of corresponding regulons. The occurrence of polar expression patterns of genes within stress-inducible operons pointed to an alternative of dynamic fine-tuning upon stress. In addition to transcriptional activation, we observed significant induction of genes at the post-transcriptional level (translatome), which identified new putative regulators and assigned genes of quorum sensing to the singlet oxygen stress response. Intriguingly, the SILAC approach explored the stress-dependent decline of photosynthetic proteins, but also identified 19 new open reading frames, which were partly validated by RNA-seq. We propose that comparative approaches as presented here will help to create multi-layered expression maps on the system level ("expressome"). Finally, intense mass spectrometry combined with RNA-seq might be the future tool of choice to re-annotate genomes in various organisms and will help to understand how they adapt to alternating conditions.show moreshow less

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Metadaten
Author: Bork A. Berghoff, Anne Konzer, Nils N. Mank, Mario Looso, Tom Rische, Konrad U. Förstner, Marcus Krüger, Gabriele Klug
URN:urn:nbn:de:bvb:20-opus-127587
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Molekulare Infektionsbiologie
Language:English
Parent Title (English):PLOS Genetics
ISSN:1553-7404
Year of Completion:2013
Volume:9
Issue:6
Pagenumber:e1003576
Source:PLoS Genetics 9(6): e1003576. doi:10.1371/journal.pgen.1003576
DOI:https://doi.org/10.1371/journal.pgen.1003576
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie
Tag:anti-sigma factor; gene expression; genome-wide analysis; in-vivo; mass spectrometry; photooxidative stress; photosynthesis genes; quanititative proteomics; rhodobacter sphaeroides; singlet oxygen stress
Release Date:2016/03/22
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung