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An Ordered NotI Fragment Map of Human Chromosome Band 11p15

Please always quote using this URN: urn:nbn:de:bvb:20-opus-45766
  • An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band llpl5. Forty-two probes, including 11 NotI-linking cosmids, were subregionaUy mapped to llpl5 using a subset of the Jl-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detectedAn ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band llpl5. Forty-two probes, including 11 NotI-linking cosmids, were subregionaUy mapped to llpl5 using a subset of the Jl-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of llp15. The predicted order of the gene loci used is cenMYODI- PTH-CALCA-ST5-RBTNI-HPX-HBB-RRMlTH/ INS!1GF2-H19-CTSD-MUC2-DRD4-HRAS-RNHtel. This map wiu allow higher resolution mapping of new Ilp15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of llp15 in clone contigs.show moreshow less

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Metadaten
Author: M. J. Higgins, N. J. Smilinich, S. Sait, A. Koenig, J. Pongratz, Manfred Gessler, C. W. Richard III., M. R. James, J. P. Sanford, B.-W. Kim, J. Cattelane, N. J. Nowak, A. Winterpacht, B. U. Zabel, D. J. Munroe, E. Bric, D. E. Housman, C. Jones, Y. Nakamura, D. S. Gerhard, T. B. Shows
URN:urn:nbn:de:bvb:20-opus-45766
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Year of Completion:1994
Source:In: GENOMICS, 23, 211-222
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
GND Keyword:Genom / Genkartierung / Genanalyse
Release Date:2010/02/10