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Differential expression of transposable elements in the medaka melanoma model
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-260615
- Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma modelMalignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.…
Autor(en): | Frederik HelmprobstORCiD, Susanne Kneitz, Barbara Klotz, Magali Naville, Corentin Dechaud, Jean-Nicolas Volff, Manfred SchartlORCiD |
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URN: | urn:nbn:de:bvb:20-opus-260615 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS One |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 16 |
Heft / Ausgabe: | 10 |
Aufsatznummer: | e0251713 |
Originalveröffentlichung / Quelle: | PLoS One (2021) 16:10, e0251713. https://doi.org/10.1371/journal.pone.0251713 |
DOI: | https://doi.org/10.1371/journal.pone.0251713 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | cancer genomics; gene prediction; genomics; human genomics; malignant tumors; melanoma; retrotransposons; transposable elements |
Datum der Freischaltung: | 31.03.2022 |
Sammlungen: | Open-Access-Publikationsfonds / Förderzeitraum 2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |