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Conventional Housing Conditions Attenuate the Development of Experimental Autoimmune Encephalomyelitis

Please always quote using this URN: urn:nbn:de:bvb:20-opus-119603
  • BACKGROUND: The etiology of multiple sclerosis (MS) has remained unclear, but a causative contribution of factors outside the central nervous system (CNS) is conceivable. It was recently suggested that gut bacteria trigger the activation of CNS-reactive T cells and the development of demyelinative disease. METHODS: C57BL/6 (B6) mice were kept either under specific pathogen free or conventional housing conditions, immunized with the myelin basic protein (MBP)-proteolipid protein (PLP) fusion protein MP4 and the development of EAE wasBACKGROUND: The etiology of multiple sclerosis (MS) has remained unclear, but a causative contribution of factors outside the central nervous system (CNS) is conceivable. It was recently suggested that gut bacteria trigger the activation of CNS-reactive T cells and the development of demyelinative disease. METHODS: C57BL/6 (B6) mice were kept either under specific pathogen free or conventional housing conditions, immunized with the myelin basic protein (MBP)-proteolipid protein (PLP) fusion protein MP4 and the development of EAE was clinically monitored. The germinal center size of the Peyer's patches was determined by immunohistochemistry in addition to the level of total IgG secretion which was assessed by ELISPOT. ELISPOT assays were also used to measure MP4-specific T cell and B cell responses in the Peyer's patches and the spleen. Ear swelling assays were performed to determine the extent of delayed-type hypersensitivity reactions in specific pathogen free and conventionally housed mice. RESULTS: In B6 mice that were actively immunized with MP4 and kept under conventional housing conditions clinical disease was significantly attenuated compared to specific pathogen free mice. Conventionally housed mice displayed increased levels of IgG secretion in the Peyer's patches, while the germinal center formation in the gut and the MP4-specific TH17 response in the spleen were diminished after immunization. Accordingly, these mice displayed an attenuated delayed type hypersensitivity (DTH) reaction in ear swelling assays. CONCLUSIONS: The data corroborate the notion that housing conditions play a substantial role in the induction of murine EAE and suggest that the presence of gut bacteria might be associated with a decreased immune response to antigens of lower affinity. This concept could be of importance for MS and calls for caution when considering the therapeutic approach to treat patients with antibiotics."show moreshow less

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Metadaten
Author: Andreas Arndt, Peter Hoffacker, Konstantin Zellmer, Oktay Goecer, Mascha S. Recks, Stefanie Kuerten
URN:urn:nbn:de:bvb:20-opus-119603
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Anatomie und Zellbiologie
Language:English
Parent Title (English):PLoS ONE
Year of Completion:2014
Volume:9
Issue:6
Pagenumber:e99794
Source:PLoS ONE 9(6): e99794. doi:10.1371/journal.pone.0099794
DOI:https://doi.org/10.1371/journal.pone.0099794
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24919069
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:B cells; Peyer's patches; T cells; enzyme-linked immunoassays; gut bacteria; immune response; multiple sclerosis; secretion
Release Date:2015/11/11
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung