Extending the Anion Channelrhodopsin-Based Toolbox for Plant Optogenetics
Please always quote using this URN: urn:nbn:de:bvb:20-opus-236617
- Optogenetics was developed in the field of neuroscience and is most commonly using light-sensitive rhodopsins to control the neural activities. Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. To extend the application of analogous optogenetic tools in the pollen tube system, weOptogenetics was developed in the field of neuroscience and is most commonly using light-sensitive rhodopsins to control the neural activities. Lately, we have expanded this technique into plant science by co-expression of a chloroplast-targeted β-carotene dioxygenase and an improved anion channelrhodopsin GtACR1 from the green alga Guillardia theta. The growth of Nicotiana tabacum pollen tube can then be manipulated by localized green light illumination. To extend the application of analogous optogenetic tools in the pollen tube system, we engineered another two ACRs, GtACR2, and ZipACR, which have different action spectra, light sensitivity and kinetic features, and characterized them in Xenopus laevis oocytes, Nicotiana benthamiana leaves and N. tabacum pollen tubes. We found that the similar molecular engineering method used to improve GtACR1 also enhanced GtACR2 and ZipACR performance in Xenopus laevis oocytes. The ZipACR1 performed in N. benthamiana mesophyll cells and N. tabacum pollen tubes with faster kinetics and reduced light sensitivity, allowing for optogenetic control of anion fluxes with better temporal resolution. The reduced light sensitivity would potentially facilitate future application in plants, grown under low ambient white light, combined with an optogenetic manipulation triggered by stronger green light.…
Author: | Yang Zhou, Meiqi Ding, Xiaodong Duan, Kai R. Konrad, Georg Nagel, Shiqiang Gao |
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URN: | urn:nbn:de:bvb:20-opus-236617 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Physiologisches Institut |
Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften | |
Language: | English |
Parent Title (English): | Membranes |
ISSN: | 2077-0375 |
Year of Completion: | 2021 |
Volume: | 11 |
Issue: | 4 |
Article Number: | 287 |
Source: | Membranes 2021, 11(4), 287; https://doi.org/10.3390/membranes11040287 |
DOI: | https://doi.org/10.3390/membranes11040287 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | light-sensitive anion channel; optogenetics; pollen tube; rhodopsin; surface potential recording |
Release Date: | 2022/01/05 |
Date of first Publication: | 2021/04/14 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |