Optogenetic control of the guard cell membrane potential and stomatal movement by the light-gated anion channel GtACR1
Please always quote using this URN: urn:nbn:de:bvb:20-opus-260925
- Guard cells control the aperture of plant stomata, which are crucial for global fluxes of CO\(_2\) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl\(^-\) and NO\(_3\)\(^-\) currents of -1 to -2 nA. The anion currents depolarizeGuard cells control the aperture of plant stomata, which are crucial for global fluxes of CO\(_2\) and water. In turn, guard cell anion channels are seen as key players for stomatal closure, but is activation of these channels sufficient to limit plant water loss? To answer this open question, we used an optogenetic approach based on the light-gated anion channelrhodopsin 1 (GtACR1). In tobacco guard cells that express GtACR1, blue- and green-light pulses elicit Cl\(^-\) and NO\(_3\)\(^-\) currents of -1 to -2 nA. The anion currents depolarize the plasma membrane by 60 to 80 mV, which causes opening of voltage-gated K+ channels and the extrusion of K+. As a result, continuous stimulation with green light leads to loss of guard cell turgor and closure of stomata at conditions that provoke stomatal opening in wild type. GtACR1 optogenetics thus provides unequivocal evidence that opening of anion channels is sufficient to close stomata.…
Author: | Shouguang Huang, Meiqi Ding, M. Rob G. Roelfsema, Ingo Dreyer, Sönke Scherzer, Khaled A. S Al-Rasheid, Shiqiang Gao, Georg Nagel, Rainer Hedrich, Kai R. Konrad |
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URN: | urn:nbn:de:bvb:20-opus-260925 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Science Advances |
Year of Completion: | 2021 |
Volume: | 7 |
Issue: | 28 |
Article Number: | eabg4619 |
Source: | Science Advances (2021) 7:28, eabg4619. DOI: 10.1126/sciadv.abg4619 |
DOI: | https://doi.org/10.1126/sciadv.abg4619 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Tag: | Arabidopsis thaliana; CO2; H+-atpase; K+ channels; R-type; SLAC1; abscisic-acid activation; intact plants; potassium channel; signal transduction |
Release Date: | 2022/04/05 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2021 |
Licence (German): | CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International |