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Lung endothelial cells strengthen, but brain endothelial cells weaken barrier properties of a human alveolar epithelium cell culture model

Please always quote using this URN: urn:nbn:de:bvb:20-opus-90284
  • The blood-air barrier in the lung consists of the alveolar epithelium, the underlying capillary endothelium, their basement membranes and the interstitial space between the cell layers. Little is known about the interactions between the alveolar and the blood compartment. The aim of the present study was to gain first insights into the possible interplay between these two neighboured cell layers. We established an in vitro Transwell model of the alveolar epithelium based on human cell line H441 and investigated the influence of conditionedThe blood-air barrier in the lung consists of the alveolar epithelium, the underlying capillary endothelium, their basement membranes and the interstitial space between the cell layers. Little is known about the interactions between the alveolar and the blood compartment. The aim of the present study was to gain first insights into the possible interplay between these two neighboured cell layers. We established an in vitro Transwell model of the alveolar epithelium based on human cell line H441 and investigated the influence of conditioned medium obtained from human lung endothelial cell line HPMEC-ST1.6R on the barrier properties of the H441 layers. As control for tissue specificity H441 layers were exposed to conditioned medium from human brain endothelial cell line hCMEC/D3. Addition of dexamethasone was necessary to obtain stable H441 cell layers. Moreover, dexamethasone increased expression of cell type I markers (caveolin-1, RAGE) and cell type II marker SP-B, whereas decreased the transepithelial electrical resistance (TEER) in a concentration dependent manner. Soluble factors obtained from the lung endothelial cell line increased the barrier significantly proven by TEER values and fluorescein permeability on the functional level and by the differential expression of tight junctional proteins on the molecular level. In contrast to this, soluble factors derived from brain endothelial cells weakened the barrier significantly. In conclusion, soluble factors from lung endothelial cells can strengthen the alveolar epithelium barrier in vitro, which suggests communication between endothelial and epithelial cells regulating the integrity of the blood-air barrier.show moreshow less

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Metadaten
Author: Winfried Neuhaus, Fabian Samwer, Steffen Kunzmann, Ralph Muellenbach, Michael Wirth, Christian P. Speer, Norbert Roewer, Carola Förster
URN:urn:nbn:de:bvb:20-opus-90284
Document Type:Journal article
Faculties:Medizinische Fakultät / Kinderklinik und Poliklinik
Medizinische Fakultät / Klinik und Poliklinik für Anästhesiologie (ab 2004)
Language:English
Parent Title (English):Differentiation
Year of Completion:2012
Volume:84
Issue:4
Pagenumber:294-304
Source:Differentiation 2012 Nov 84(4):294-304. doi: 10.1016/j.diff.2012.08.006
URL:http://www.sciencedirect.com/science/article/pii/S0301468112001144
DOI:https://doi.org/10.1016/j.diff.2012.08.006
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=23023065
Sonstige beteiligte Institutionen:Department of Medicinal Chemistry, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
Sonstige beteiligte Institutionen:Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
Sonstige beteiligte Institutionen:Comprehensive Heart Failure Center (CHFC) Würzburg, University Hospital Würzburg, Straubmühlweg 2a, 97078 Wuerzburg, Germany
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:alveolar epithelium in vitro model, claudin-1, claudin-3, claudin-4, claudin-5
Release Date:2014/01/21
EU-Project number / Contract (GA) number:241778
OpenAIRE:OpenAIRE
Licence (German):License LogoDeutsches Urheberrecht