Normal Platelet Integrin Function in Mice Lacking Hydrogen Peroxide-Induced Clone-5 (Hic-5)
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-125724
- Integrin αIIbβ3 plays a central role in the adhesion and aggregation of platelets and thus is essential for hemostasis and thrombosis. Integrin activation requires the transmission of a signal from the small cytoplasmic tails of the α or β subunit to the large extracellular domains resulting in conformational changes of the extracellular domains to enable ligand binding. Hydrogen peroxide-inducible clone-5 (Hic-5), a member of the paxillin family, serves as a focal adhesion adaptor protein associated with αIIbβ3 at its cytoplasmic tails.Integrin αIIbβ3 plays a central role in the adhesion and aggregation of platelets and thus is essential for hemostasis and thrombosis. Integrin activation requires the transmission of a signal from the small cytoplasmic tails of the α or β subunit to the large extracellular domains resulting in conformational changes of the extracellular domains to enable ligand binding. Hydrogen peroxide-inducible clone-5 (Hic-5), a member of the paxillin family, serves as a focal adhesion adaptor protein associated with αIIbβ3 at its cytoplasmic tails. Previous studies suggested Hic-5 as a novel regulator of integrin αIIbβ3 activation and platelet aggregation in mice. To assess this in more detail, we generated Hic-5-null mice and analyzed activation and aggregation of their platelets in vitro and in vivo. Surprisingly, lack of Hic-5 had no detectable effect on platelet integrin activation and function in vitro and in vivo under all tested conditions. These results indicate that Hic-5 is dispensable for integrin αIIbβ3 activation and consequently for arterial thrombosis and hemostasis in mice.…
Autor(en): | Michael Popp, Ina Thielman, Bernhard Nieswandt, David Stegner |
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URN: | urn:nbn:de:bvb:20-opus-125724 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Rudolf-Virchow-Zentrum |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS One |
Erscheinungsjahr: | 2015 |
Band / Jahrgang: | 10 |
Heft / Ausgabe: | 7 |
Seitenangabe: | e0133429 |
Originalveröffentlichung / Quelle: | PLoS ONE 10(7): e0133429. doi:10.1371/journal. pone.0133429 |
DOI: | https://doi.org/10.1371/journal.pone.0133429 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | blood; fibrinogen; flow cytometry; integrins; platelet activation; platelet aggregation; platelets; thrombin |
Datum der Freischaltung: | 02.02.2016 |
Sammlungen: | Open-Access-Publikationsfonds / Förderzeitraum 2015 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |