Doxycycline Inducible Melanogenic Vaccinia Virus as Theranostic Anti-Cancer Agent
Please always quote using this URN: urn:nbn:de:bvb:20-opus-124987
- We reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouseWe reported earlier the diagnostic potential of a melanogenic vaccinia virus based system in magnetic resonance (MRI) and optoacoustic deep tissue imaging (MSOT). Since melanin overproduction lead to attenuated virus replication, we constructed a novel recombinant vaccinia virus strain (rVACV), GLV-1h462, which expressed the key enzyme of melanogenesis (tyrosinase) under the control of an inducible promoter-system. In this study melanin production was detected after exogenous addition of doxycycline in two different tumor xenograft mouse models. Furthermore, it was confirmed that this novel vaccinia virus strain still facilitated signal enhancement as detected by MRI and optoacoustic tomography. At the same time we demonstrated an enhanced oncolytic potential compared to the constitutively melanin synthesizing rVACV system.…
Author: | Lorenz Kirscher, Xosé Luis Deán-Ben, Miriam Scadeng, Angelika Zaremba, Qian Zhang, Christina Kober, Thomas Felix Fehm, Daniel Razansky, Vasilis Ntziachristos, Jochen Stritzker, Aladar A. Szalay |
---|---|
URN: | urn:nbn:de:bvb:20-opus-124987 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie |
Language: | English |
Parent Title (English): | Theranostics |
Year of Completion: | 2015 |
Volume: | 5 |
Issue: | 10 |
Pagenumber: | 1045-1057 |
Source: | Theranostics 2015; 5(10): 1045-1057. doi: 10.7150/thno.12533 |
DOI: | https://doi.org/10.7150/thno.12533 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | molecular imaging; oncolysis; reporter gene; virotherapy |
Release Date: | 2016/02/02 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2015 |
Licence (German): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitung |