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Construction of a high titer Infectious HIV-1 subtype C proviral clone from South Africa

Please always quote using this URN: urn:nbn:de:bvb:20-opus-76340
  • The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. WeThe Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.show moreshow less

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Metadaten
Author: Graeme Jacobs, Stefanie Bock, Anita Schuch, Rebecca Moschall, Eva-Maria Schrom, Juliane Zahn, Christian Reuter, Wolfgang Preiser, Axel Rethwilm, Susan Engelbrecht, Thomas Krekau, Jochen Bodem
URN:urn:nbn:de:bvb:20-opus-76340
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Virologie und Immunbiologie
Language:English
Year of Completion:2012
Source:Viruses (2012) 4: 9, 1830-1843, doi:10.3390/v4091830
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
GND Keyword:HIV
Tag:HIV-1; subtype C; proviral plasmid; viral replication; resistance assays; Vpu; CD317; CD4
Release Date:2013/07/16
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2012
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung