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Magnetic Resonance Imaging of Tumors Colonized with Bacterial Ferritin-Expressing \(Escherichia\) \(coli\)

Please always quote using this URN: urn:nbn:de:bvb:20-opus-140920
  • Background: Recent studies have shown that human ferritin can be used as a reporter of gene expression for magnetic resonance imaging (MRI). Bacteria also encode three classes of ferritin-type molecules with iron accumulation properties. Methods and Findings: Here, we investigated whether these bacterial ferritins can also be used as MRI reporter genes and which of the bacterial ferritins is the most suitable reporter. Bacterial ferritins were overexpressed in probiotic E. coli Nissle 1917. Cultures of these bacteria were analyzedBackground: Recent studies have shown that human ferritin can be used as a reporter of gene expression for magnetic resonance imaging (MRI). Bacteria also encode three classes of ferritin-type molecules with iron accumulation properties. Methods and Findings: Here, we investigated whether these bacterial ferritins can also be used as MRI reporter genes and which of the bacterial ferritins is the most suitable reporter. Bacterial ferritins were overexpressed in probiotic E. coli Nissle 1917. Cultures of these bacteria were analyzed and those generating highest MRI contrast were further investigated in tumor bearing mice. Among members of three classes of bacterial ferritin tested, bacterioferritin showed the most promise as a reporter gene. Although all three proteins accumulated similar amounts of iron when overexpressed individually, bacterioferritin showed the highest contrast change. By site-directed mutagenesis we also show that the heme iron, a unique part of the bacterioferritin molecule, is not critical for MRI contrast change. Tumor-specific induction of bacterioferritin-expression in colonized tumors resulted in contrast changes within the bacteria-colonized tumors. Conclusions: Our data suggest that colonization and gene expression by live vectors expressing bacterioferritin can be monitored by MRI due to contrast changes.show moreshow less

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Metadaten
Author: Philip J. Hill, Jochen Stritzker, Miriam Scadeng, Ulrike Geissinger, Daniel Haddad, Thomas C. Basse-Lüsebrink, Uwe Gbureck, Peter Jakob, Aladar A. Szalay
URN:urn:nbn:de:bvb:20-opus-140920
Document Type:Journal article
Faculties:Fakultät für Physik und Astronomie / Physikalisches Institut
Medizinische Fakultät / Institut für Molekulare Infektionsbiologie
Fakultät für Biologie / Rudolf-Virchow-Zentrum
Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie
Language:English
Parent Title (English):PLoS ONE
Year of Completion:2011
Volume:6
Issue:10
Pagenumber:e25409
Source:PLoS ONE 6(10): e25409. doi:10.1371/journal.pone.0025409
DOI:https://doi.org/10.1371/journal.pone.0025409
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 616 Krankheiten
Tag:Blood-brain barrier; Breast-tumors; Gene-expression; Iron-uptake; MRI reporter; Mice; Proteins; Salmonella-typhimurium; Sugar-transport; Therapy
Release Date:2018/11/20
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung