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miRNAs can be generally associated with human pathologies as exemplified for miR-144*

Please always quote using this URN: urn:nbn:de:bvb:20-opus-114349
  • Background: miRNA profiles are promising biomarker candidates for a manifold of human pathologies, opening new avenues for diagnosis and prognosis. Beyond studies that describe miRNAs frequently as markers for specific traits, we asked whether a general pattern for miRNAs across many diseases exists. Methods: We evaluated genome-wide circulating profiles of 1,049 patients suffering from 19 different cancer and non-cancer diseases as well as unaffected controls. The results were validated on 319 individuals using qRT-PCR. Results: WeBackground: miRNA profiles are promising biomarker candidates for a manifold of human pathologies, opening new avenues for diagnosis and prognosis. Beyond studies that describe miRNAs frequently as markers for specific traits, we asked whether a general pattern for miRNAs across many diseases exists. Methods: We evaluated genome-wide circulating profiles of 1,049 patients suffering from 19 different cancer and non-cancer diseases as well as unaffected controls. The results were validated on 319 individuals using qRT-PCR. Results: We discovered 34 miRNAs with strong disease association. Among those, we found substantially decreased levels of hsa-miR-144* and hsa-miR-20b with AUC of 0.751 ( 95% CI: 0.703-0.799), respectively. We also discovered a set of miRNAs, including hsa-miR-155*, as rather stable markers, offering reasonable control miRNAs for future studies. The strong downregulation of hsa-miR-144* and the less variable pattern of hsa-miR-155* has been validated in a cohort of 319 samples in three different centers. Here, breast cancer as an additional disease phenotype not included in the screening phase has been included as the 20th trait. Conclusions: Our study on 1,368 patients including 1,049 genome-wide miRNA profiles and 319 qRT-PCR validations further underscores the high potential of specific blood-borne miRNA patterns as molecular biomarkers. Importantly, we highlight 34 miRNAs that are generally dysregulated in human pathologies. Although these markers are not specific to certain diseases they may add to the diagnosis in combination with other markers, building a specific signature. Besides these dysregulated miRNAs, we propose a set of constant miRNAs that may be used as control markers.show moreshow less

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Metadaten
Author: Andreas Keller, Petra Leidinger, Britta Vogel, Christina Backes, Abdou ElSharawy, Valentina Galata, Sabine C. Mueller, Sabine Marquart, Michael G. Schrauder, Reiner Strick, Andrea Bauer, Jörg Wischhusen, Markus Beier, Jochen Kohlhaas, Hugo A. Katus, Jörg Hoheisel, Andre Franke, Benjamin Meder, Eckart Meese
URN:urn:nbn:de:bvb:20-opus-114349
Document Type:Journal article
Faculties:Medizinische Fakultät / Frauenklinik und Poliklinik
Language:English
Parent Title (English):BMC MEDICINE
ISSN:1741-7015
Year of Completion:2014
Volume:12
Pagenumber:224
Source:BMC Medicine 2014, 12:224. DOI 10.1186/s12916-014-0224-0
DOI:https://doi.org/10.1186/s12916-014-0224-0
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/PMC4268797
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:bioinformatics; biomarker; disease; expression; gene; lung-cancer; miRNA; microarray; microna profiles; multiple sclerosis; peripheral blood; serum; signature
Release Date:2015/06/29
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung