The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 3 of 4
Back to Result List

Toxicity and functional impairment in human adipose tissue-derived stromal cells (hASCs) following long-term exposure to very small iron oxide particles (VSOPs)

Please always quote using this URN: urn:nbn:de:bvb:20-opus-203676
  • Magnetic nanoparticles (NPs), such as very small iron oxide NPs (VSOPs) can be used for targeted drug delivery, cancer treatment or tissue engineering. Another important field of application is the labelling of mesenchymal stem cells to allow in vivo tracking and visualization of transplanted cells using magnetic resonance imaging (MRI). For these NPs, however, various toxic effects, as well as functional impairment of the exposed cells, are described. The present study evaluates the influence of VSOPs on the multilineage differentiationMagnetic nanoparticles (NPs), such as very small iron oxide NPs (VSOPs) can be used for targeted drug delivery, cancer treatment or tissue engineering. Another important field of application is the labelling of mesenchymal stem cells to allow in vivo tracking and visualization of transplanted cells using magnetic resonance imaging (MRI). For these NPs, however, various toxic effects, as well as functional impairment of the exposed cells, are described. The present study evaluates the influence of VSOPs on the multilineage differentiation ability and cytokine secretion of human adipose tissue derived stromal cells (hASCs) after long-term exposure. Human ASCs were labelled with VSOPs, and the efficacy of the labelling was documented over 4 weeks in vitro cultivation of the labelled cells. Unlabelled hASCs served as negative controls. Four weeks after labelling, adipogenic and osteogenic differentiation was histologically evaluated and quantified by polymerase chain reaction (PCR). Changes in gene expression of IL-6, IL-8, VEGF and caspase 3 were determined over 4 weeks. Four weeks after the labelling procedure, labelled and unlabelled hASCs did not differ in the gene expression of IL-6, IL-8, VEGF and caspase 3. Furthermore, the labelling procedure had no influence on the multidifferentiation ability of hASC. The percentage of labelled cells decreased during in vitro expansion over 4 weeks. Labelling with VSOPs and long-term intracellular disposition probably have no influence on the physiological functions of hASCs. This could be important for the future in vivo use of iron oxide NPs.show moreshow less

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar Statistics
Metadaten
Author: Katrin Radeloff, Andreas Radeloff, Mario Ramos Tirado, Agmal Scherzad, Rudolf Hagen, Norbert H. Kleinsasser, Stephan Hackenberg
URN:urn:nbn:de:bvb:20-opus-203676
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen
Language:English
Parent Title (English):Nanomaterials
ISSN:2079-4991
Year of Completion:2020
Volume:10
Issue:4
Article Number:741
Source:Nanomaterials (2020) 10:4, 741. https://doi.org/10.3390/nano10040741
DOI:https://doi.org/10.3390/nano10040741
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:VSOP; differentiation potential; human adipose-derived stromal cells; iron oxide nanoparticles; long-term exposure; nanoparticles; stem cells; toxicity
Release Date:2022/06/07
Date of first Publication:2020/04/13
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International