Molecular cloning of the genome of human spumaretrovirus
Please always quote using this URN: urn:nbn:de:bvb:20-opus-61518
- DNA ofhuman spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage A and bacterial plasmid vectors. The recombinant plasm.ids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained from viral DNA. The recombinant clones isolated contained viral DNA inserts which rangein size from 2.2 kb to 15.4 kb. The recombinant clones allowed to construct a physical map of the complete HSRVDNA ofhuman spumaretrovirus (HSRV) was cloned from both cDNA and from viral DNA into phage A and bacterial plasmid vectors. The recombinant plasm.ids harboring viral DNA were characterized by Southern blot hybridization and restriction mapping. Physical maps were constructed from cDNA and found to be colinear with the restriction maps obtained from viral DNA. The recombinant clones isolated contained viral DNA inserts which rangein size from 2.2 kb to 15.4 kb. The recombinant clones allowed to construct a physical map of the complete HSRV provirus of 12.2 kb.…
| Author: | Axel Rethwilm, G. Darai, A. Rösen, Bernd Maurer, Rolf M. Flügel |
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| URN: | urn:nbn:de:bvb:20-opus-61518 |
| Document Type: | Journal article |
| Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
| Language: | English |
| Year of Completion: | 1987 |
| Source: | In: Gene (1987) 59, 1, 19-28 |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| GND Keyword: | Virologie |
| Release Date: | 2011/09/17 |
| Licence (German): |  Deutsches Urheberrecht |