Correlative Analysis of Intra– Versus Extracellular Cell Detachment Events via the Alignment of Optical Imaging and Detachment Force Quantification
Please always quote using this URN: urn:nbn:de:bvb:20-opus-318544
- In recent decades, hybrid characterization systems have become pillars in the study of cellular biomechanics. Especially, Atomic Force Microscopy (AFM) is combined with a variety of optical microscopy techniques to discover new aspects of cell adhesion. AFM, however, is limited to the early-stage of cell adhesion, so that the forces of mature cell contacts cannot be addressed. Even though the invention of Fluidic Force Microscopy (FluidFM) overcomes these limitations by combining the precise force-control of AFM with microfluidics, theIn recent decades, hybrid characterization systems have become pillars in the study of cellular biomechanics. Especially, Atomic Force Microscopy (AFM) is combined with a variety of optical microscopy techniques to discover new aspects of cell adhesion. AFM, however, is limited to the early-stage of cell adhesion, so that the forces of mature cell contacts cannot be addressed. Even though the invention of Fluidic Force Microscopy (FluidFM) overcomes these limitations by combining the precise force-control of AFM with microfluidics, the correlative investigation of detachment forces arising from spread mammalian cells has been barely achieved. Here, a novel multifunctional device integrating Fluorescence Microscopy (FL) into FluidFM technology (FL-FluidFM) is introduced, enabling real-time optical tracking of entire cell detachment processes in parallel to the undisturbed acquisition of force-distance curves. This setup, thus, allows for entailing two pieces of information at once. As proof-of-principle experiment, this method is applied to fluorescently labeled rat embryonic fibroblast (REF52) cells, demonstrating a precise matching between identified force-jumps and visualized cellular unbinding steps. This study, thus, presents a novel characterization tool for the correlated evaluation of mature cell adhesion, which has great relevance, for instance, in the development of biomaterials or the fight against diseases such as cancer.…
Author: | Franziska Weigl, Carina Blum, Ana Sancho, Jürgen Groll |
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URN: | urn:nbn:de:bvb:20-opus-318544 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde |
Language: | English |
Parent Title (English): | Advanced Materials Technologies |
ISSN: | 2365-709X |
Year of Completion: | 2022 |
Volume: | 7 |
Issue: | 11 |
Article Number: | 2200195 |
Source: | Advanced Materials Technologies 2022, 7(11):2200195. DOI: 10.1002/admt.202200195 |
DOI: | https://doi.org/10.1002/admt.202200195 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | FluidFM technology; Fluorescence Microscopy; detachment force quantification |
Release Date: | 2023/07/18 |
Licence (German): | CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International |