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RNase E cleavage shapes the transcriptome of Rhodobacter sphaeroides and strongly impacts phototrophic growth
Please always quote using this URN: urn:nbn:de:bvb:20-opus-177139
- Bacteria adapt to changing environmental conditions by rapid changes in their transcriptome. This is achieved not only by adjusting rates of transcription but also by processing and degradation of RNAs. We applied TIER-Seq (transiently inactivating an endoribonuclease followed by RNA-Seq) for the transcriptome-wide identification of RNase E cleavage sites and of 5′ RNA ends, which are enriched when RNase E activity is reduced in Rhodobacter sphaeroides. These results reveal the importance of RNase E for the maturation and turnover of mRNAs,Bacteria adapt to changing environmental conditions by rapid changes in their transcriptome. This is achieved not only by adjusting rates of transcription but also by processing and degradation of RNAs. We applied TIER-Seq (transiently inactivating an endoribonuclease followed by RNA-Seq) for the transcriptome-wide identification of RNase E cleavage sites and of 5′ RNA ends, which are enriched when RNase E activity is reduced in Rhodobacter sphaeroides. These results reveal the importance of RNase E for the maturation and turnover of mRNAs, rRNAs, and sRNAs in this guanine-cytosine-rich α-proteobacterium, some of the latter have well-described functions in the oxidative stress response. In agreement with this, a role of RNase E in the oxidative stress response is demonstrated. A remarkably strong phenotype of a mutant with reduced RNase E activity was observed regarding the formation of photosynthetic complexes and phototrophic growth, whereas there was no effect on chemotrophic growth.…
Author: | Konrad U FörstnerORCiD, Carina M ReuscherORCiD, Kerstin Haberzettl, Lennart Weber, Gabriele KlugORCiD |
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URN: | urn:nbn:de:bvb:20-opus-177139 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Language: | English |
Parent Title (English): | Life Science Alliance |
Year of Completion: | 2018 |
Volume: | 1 |
Issue: | 4 |
Pagenumber: | e201800080 |
Source: | Life Science Alliance (2018) 1(4):e201800080. DOI: 10.26508/lsa.201800080 |
DOI: | https://doi.org/10.26508/lsa.201800080 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | RNase E; Rhodobacter sphaeroides; phototrophic growth |
Release Date: | 2019/04/05 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2018 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |