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Expression of monoamine transporters, nitric oxide synthase 3, and neurotrophin genes in antidepressant-stimulated astrocytes

Please always quote using this URN: urn:nbn:de:bvb:20-opus-123627
  • Background: There is increasing evidence that glial cells play a role in the pathomechanisms of mood disorders and the mode of action of antidepressant drugs. Methods: To examine whether there is a direct effect on the expression of different genes encoding proteins that have been implicated in the pathophysiology of affective disorders, primary astrocyte cell cultures from rats were treated with two different antidepressant drugs, imipramine and escitalopram, and the RNA expression of brain-derived neurotrophic factor (Bdnf), serotoninBackground: There is increasing evidence that glial cells play a role in the pathomechanisms of mood disorders and the mode of action of antidepressant drugs. Methods: To examine whether there is a direct effect on the expression of different genes encoding proteins that have been implicated in the pathophysiology of affective disorders, primary astrocyte cell cultures from rats were treated with two different antidepressant drugs, imipramine and escitalopram, and the RNA expression of brain-derived neurotrophic factor (Bdnf), serotonin transporter (5Htt), dopamine transporter (Dat), and endothelial nitric oxide synthase (Nos3) was examined. Results: Stimulation of astroglial cell culture with imipramine, a tricyclic antidepressant, led to a significant increase of the Bdnf RNA level whereas treatment with escitalopram did not. In contrast, 5Htt was not differentially expressed after antidepressant treatment. Finally, neither Dat nor Nos3 RNA expression was detected in cultured astrocytes. Conclusion: These data provide further evidence for a role of astroglial cells in the molecular mechanisms of action of antidepressants.show moreshow less

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Metadaten
Author: Sarah Kittel-Schneider, Gunter Kenis, Julia Schek, Daniel van den Hove, Jos Prickaerts, Klaus-Peter Lesch, Harry Steinbusch, Andreas Reif
URN:urn:nbn:de:bvb:20-opus-123627
Document Type:Journal article
Faculties:Medizinische Fakultät / Klinik und Poliklinik für Psychiatrie, Psychosomatik und Psychotherapie
Language:English
Parent Title (English):Frontiers in Psychiatry
Year of Completion:2012
Volume:3
Pagenumber:33
Source:Frontiers in Psychiatry 3:33. doi:10.3389/fpsyt.2012.00033
DOI:https://doi.org/10.3389/fpsyt.2012.00033
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=PMC3330247
Dewey Decimal Classification:1 Philosophie und Psychologie / 15 Psychologie / 150 Psychologie
Tag:BDNF; antidepressant; astrocytes; depression; geneexpression; glia; mechanismofaction; monoamine transporters; nitricoxidesynthase
Release Date:2015/12/22
Licence (German):License LogoCC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell