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Low energy status under methionine restriction is essentially independent of proliferation or cell contact inhibition

Please always quote using this URN: urn:nbn:de:bvb:20-opus-262329
  • Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929 responds to methionine restriction (MetR) for a short time period with LEM at the metabolic level defined by a characteristic fingerprint consisting of the molecules acetoacetate, creatine, spermidine, GSSG, UDP-glucose, pantothenate, and ATP. Here, we used mass spectrometry (LC/MS) to investigate the influence of proliferation and contact inhibition on the energy status of cells. Interestingly, the energy status was essentially independent of proliferation or contact inhibition. LC/MS analyses showed that in full medium, the cells maintain active and energetic metabolism for optional proliferation. In contrast, MetR induced LEM independently of proliferation or contact inhibition. These results are important for cell behaviour under MetR and for the optional application of restrictions in cancer therapy.show moreshow less

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Metadaten
Author: Corinna Koderer, Werner Schmitz, Anna Chiara Wünsch, Julia Balint, Mohamed El-Mesery, Julian Manuel Volland, Stefan Hartmann, Christian Linz, Alexander Christian Kübler, Axel Seher
URN:urn:nbn:de:bvb:20-opus-262329
Document Type:Journal article
Faculties:Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften
Medizinische Fakultät / Klinik und Poliklinik für Mund-, Kiefer- und Plastische Gesichtschirurgie
Language:English
Parent Title (English):Cells
ISSN:2073-4409
Year of Completion:2022
Volume:11
Issue:3
Article Number:551
Source:Cells (2022) 11:3, 551. https://doi.org/10.3390/cells11030551
DOI:https://doi.org/10.3390/cells11030551
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:L929; LC/MS; amino acid; caloric restriction; contact inhibition; liquid chromatography/mass spectrometry; mass spectrometry; metabolomics; methionine restriction; proliferation
Release Date:2023/02/03
Date of first Publication:2022/02/04
Open-Access-Publikationsfonds / Förderzeitraum 2022
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International