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Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain

Please always quote using this URN: urn:nbn:de:bvb:20-opus-137957
  • The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of theThe Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.show moreshow less

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Metadaten
Author: Halder Partho, Yi-chun Chen, Janine Brauckhoff, Alois Hofbauer, Marie-Christine Dabauvalle, Urs Lewandrowski, Christiane Winkler, Albert Sickmann, Erich Buchner
URN:urn:nbn:de:bvb:20-opus-137957
Document Type:Journal article
Faculties:Medizinische Fakultät / Institut für Klinische Neurobiologie
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Fakultät für Biologie / Rudolf-Virchow-Zentrum
Language:English
Parent Title (English):PLoS One
Year of Completion:2011
Volume:6
Issue:12
Pagenumber:e29352
Source:PLoS ONE 6(12): e29352. doi:10.1371/journal.pone.0029352
DOI:https://doi.org/10.1371/journal.pone.0029352
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 595 Arthropoden (Gliederfüßer)
Tag:antigen processing and recognition; drosophila melanogaster; gel electrophoresis; hybridomas; immunohistochemistry techniques; immunoprecipitation; neuropil; silver staining
Release Date:2016/08/29
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2011
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung