P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress
Please always quote using this URN: urn:nbn:de:bvb:20-opus-221726
- DNA damage response (DDR) involves dramatic transcriptional alterations, the mechanisms of which remain ill defined. Here, we show that following genotoxic stress, the RNA-binding motif protein 7 (RBM7) stimulates RNA polymerase II (Pol II) transcription and promotes cell viability by activating the positive transcription elongation factor b (P-TEFb) via its release from the inhibitory 7SK small nuclear ribonucleoprotein (7SK snRNP). This is mediated by activation of p38MAPK, which triggers enhanced binding of RBM7 with core subunits of 7SKDNA damage response (DDR) involves dramatic transcriptional alterations, the mechanisms of which remain ill defined. Here, we show that following genotoxic stress, the RNA-binding motif protein 7 (RBM7) stimulates RNA polymerase II (Pol II) transcription and promotes cell viability by activating the positive transcription elongation factor b (P-TEFb) via its release from the inhibitory 7SK small nuclear ribonucleoprotein (7SK snRNP). This is mediated by activation of p38MAPK, which triggers enhanced binding of RBM7 with core subunits of 7SK snRNP. In turn, P-TEFb relocates to chromatin to induce transcription of short units, including key DDR genes and multiple classes of non-coding RNAs. Critically, interfering with the axis of RBM7 and P-TEFb provokes cellular hypersensitivity to DNA-damage-inducing agents due to activation of apoptosis. Our work uncovers the importance of stress-dependent stimulation of Pol II pause release, which enables a pro-survival transcriptional response that is crucial for cell fate upon genotoxic insult.…
Author: | Andrii Bugai, Alexandre J. C. Quaresma, Caroline C. Friedel, Tina Lenasi, Robert Düster, Christopher R. Sibley, Koh Fujinaga, Petra Kukanja, Thomas Hennig, Melanie Blasius, Matthias Geyer, Jernej Ule, Lars Dölken, Matjaž Barborič |
---|---|
URN: | urn:nbn:de:bvb:20-opus-221726 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Virologie und Immunbiologie |
Language: | English |
Parent Title (English): | Molecular Cell |
Year of Completion: | 2019 |
Volume: | 74 |
Pagenumber: | 254-267 |
Source: | Molecular Cell (2019) 74:254-267. https://doi.org/10.1016/j.molcel.2019.01.033 |
DOI: | https://doi.org/10.1016/j.molcel.2019.01.033 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | 7SK snRNP; CDK9; DNA damage response; P-TEFb; Pol II elongation; Pol II pause release; RBM7; apoptosis; genotoxic stress; p38 MAP kinase |
Release Date: | 2024/08/21 |
EU-Project number / Contract (GA) number: | 206726 |
OpenAIRE: | OpenAIRE |
Licence (German): | ![]() |