Evaluation of the Neurogenic Potential in the Rat Inferior Colliculus from Early Postnatal Days Until Adulthood
Please always quote using this URN: urn:nbn:de:bvb:20-opus-235811
- Neural stem cells (NSCs) have been recently identified in the inferior colliculus (IC). These cells are of particular interest, as no casual therapeutic options for impaired neural structures exist. This research project aims to evaluate the neurogenic potential in the rat IC from early postnatal days until adulthood. The IC of rats from postnatal day 6 up to 48 was examined by neurosphere assays and histological sections. In free-floating IC cell cultures, neurospheres formed from animals from early postnatal to adulthood. The amount ofNeural stem cells (NSCs) have been recently identified in the inferior colliculus (IC). These cells are of particular interest, as no casual therapeutic options for impaired neural structures exist. This research project aims to evaluate the neurogenic potential in the rat IC from early postnatal days until adulthood. The IC of rats from postnatal day 6 up to 48 was examined by neurosphere assays and histological sections. In free-floating IC cell cultures, neurospheres formed from animals from early postnatal to adulthood. The amount of generated neurospheres decreased in older ages and increased with the number of cell line passages. Cells in the neurospheres and the histological sections stained positively with NSC markers (Doublecortin, Sox-2, Musashi-1, Nestin, and Atoh1). Dissociated single cells from the neurospheres differentiated and were stained positively for the neural lineage markers β-III-tubulin, glial fibrillary acidic protein, and myelin basic protein. In addition, NSC markers (Doublecortin, Sox-2, CDK5R1, and Ascl-1) were investigated by qRT-PCR. In conclusion, a neurogenic potential in the rat IC was detected and evaluated from early postnatal days until adulthood. The identification of NSCs in the rat IC and their age-specific characteristics contribute to a better understanding of the development and the plasticity of the auditory pathway and might be activated for therapeutic use.…
| Author: | Jonas Engert, Kristen Rak, Linsa Bieniussa, Miriam Scholl, Rudolf Hagen, Johannes Völker |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-235811 |
| Document Type: | Journal article |
| Faculties: | Medizinische Fakultät / Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen |
| Language: | English |
| Parent Title (English): | Molecular Neurobiology |
| ISSN: | 0893-7648 |
| Year of Completion: | 2021 |
| Volume: | 58 |
| Pagenumber: | 719–734 |
| Source: | Molecular Neurobiology (2021) 58:719–734. https://doi.org/10.1007/s12035-020-02151-6 |
| DOI: | https://doi.org/10.1007/s12035-020-02151-6 |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Tag: | central auditory pathway; inferior colliculus; neural progenitor cells; neural stem cells; neurosphere |
| Release Date: | 2021/06/28 |
| Licence (German): |  CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |