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Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp EG49 and Nocardiopsis sp RV163

Please always quote using this URN: urn:nbn:de:bvb:20-opus-116547
  • Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of eitherTwo sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by H-1 NMR. Ten known compounds, including angucycline, diketopiperazine and beta-carboline derivatives 1-10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a, 6,11a, 12-tetrahydro-5a, 11a-dimethyl[1,4]benzoxazino[3,2-b][1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes.show moreshow less

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Metadaten
Author: Yousef Dashti, Tanja Grkovic, Usama Ramadan Abdelmohsen, Ute Hentschel, Ronald J. Quinn
URN:urn:nbn:de:bvb:20-opus-116547
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Parent Title (English):MARINE DRUGS
ISSN:1660-3397
Year of Completion:2014
Volume:12
Issue:5
Pagenumber:3046-3059
Source:Marine Drugs 2014, 12, 3046-3059; doi:10.3390/md12053046
DOI:https://doi.org/10.3390/md12053046
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24857962
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 593 Wirbellose des Meeres und der Meeresküste
Tag:A-D; NMR fingerprint; aspergillus fumigatus; bioactivity; biosynthesis; co-cultivation; induced metabolites; marine; natural products; sponge-associated actinomyetes
Release Date:2015/07/30
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung