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Dereplication Strategies for Targeted Isolation of New Antitrypanosomal Actinosporins A and B from a Marine Sponge Associated-Actinokineospora sp EG49

Please always quote using this URN: urn:nbn:de:bvb:20-opus-119876
  • High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complementary metabolomic tools to dereplicate the chemical profile of the new and antitrypanosomally active sponge-associated bacterium Actinokineospora sp. EG49 extract. Principal Component (PCA), hierarchical clustering (HCA), and orthogonal partial least square-discriminant analysis (OPLS-DA) were used to evaluate the HRFTMS and NMR data of crude extracts from four different fermentation approaches. StatisticalHigh resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complementary metabolomic tools to dereplicate the chemical profile of the new and antitrypanosomally active sponge-associated bacterium Actinokineospora sp. EG49 extract. Principal Component (PCA), hierarchical clustering (HCA), and orthogonal partial least square-discriminant analysis (OPLS-DA) were used to evaluate the HRFTMS and NMR data of crude extracts from four different fermentation approaches. Statistical analysis identified the best culture one-strain-many-compounds (OSMAC) condition and extraction procedure, which was used for the isolation of novel bioactive metabolites. As a result, two new O-glycosylated angucyclines, named actinosporins A (1) and B (2), were isolated from the broth culture of Actinokineospora sp. strain EG49, which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structures of actinosporins A and B were determined by 1D- and 2D-NMR techniques, as well as high resolution tandem mass spectrometry. Testing for antiparasitic properties showed that actinosporin A exhibited activity against Trypanosoma brucei brucei with an IC₅₀ value of 15 µM; however no activity was detected against Leishmania major and Plasmodium falciparum, therefore suggesting its selectivity against the parasite Trypanosoma brucei brucei; the causative agent of sleeping sickness.show moreshow less

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Metadaten
Author: Usama Ramadan Abdelmohsen, Cheng Cheng, Christina Viegelmann, Tong Zhang, Tanja Grkovic, Safwat Ahmed, Ronald J. Quinn, Ute Hentschel, RuAngelie Edrada-Ebel
URN:urn:nbn:de:bvb:20-opus-119876
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Parent Title (English):Marine Drugs
ISSN:1660-3397
Year of Completion:2014
Volume:12
Issue:3
Pagenumber:1220-44
Source:Marine Drugs 2014, 12, 1220-1244; doi:10.3390/md12031220
DOI:https://doi.org/10.3390/md12031220
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24663112
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 593 Wirbellose des Meeres und der Meeresküste
Tag:Actinokineospora; Spheciospongia vagabunda; actinosporins; anti-trypanosoma; dereplication; secondary metabolomics
Release Date:2015/11/17
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung