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Actinomycetes from Red Sea Sponges: Sources for Chemical and Phylogenetic Diversity

Please always quote using this URN: urn:nbn:de:bvb:20-opus-112882
  • The diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-basedThe diversity of actinomycetes associated with marine sponges collected off Fsar Reef (Saudi Arabia) was investigated in the present study. Forty-seven actinomycetes were cultivated and phylogenetically identified based on 16S rRNA gene sequencing and were assigned to 10 different actinomycete genera. Eight putatively novel species belonging to genera Kocuria, Mycobacterium, Nocardia, and Rhodococcus were identified based on sequence similarity values below 98.2% to other 16S rRNA gene sequences available in the NCBI database. PCR-based screening for biosynthetic genes including type I and type II polyketide synthases (PKS-I, PKS-II) as well as nonribosomal peptide synthetases (NRPS) showed that 20 actinomycete isolates encoded each at least one type of biosynthetic gene. The organic extracts of nine isolates displayed bioactivity against at least one of the test pathogens, which were Gram-positive and Gram-negative bacteria, fungi, human parasites, as well as in a West Nile Virus protease enzymatic assay. These results emphasize that marine sponges are a prolific resource for novel bioactive actinomycetes with potential for drug discovery.show moreshow less

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Metadaten
Author: Usama Ramadan Abdelmohsen, Chen Yang, Hannes Horn, Dina Hajjar, Timothy Ravasi, Ute Hentschel
URN:urn:nbn:de:bvb:20-opus-112882
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Year of Completion:2014
Source:Marine Drugs 2014, 12, 2771-2789; doi:10.3390/md12052771
DOI:https://doi.org/10.3390/md12052771
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 593 Wirbellose des Meeres und der Meeresküste
GND Keyword:Meeresschwämme
Tag:NRPS; PKS I; PKS II; Red sea; actinomycetes; bioactivity; sponges
Release Date:2015/05/15
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2014
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung