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Comparative proteome analysis of Milnesium tardigradum in early embryonic state versus adults in active and anhydrobiotic state
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-134447
- Tardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with aTardigrades have fascinated researchers for more than 300 years because of their extraordinary capability to undergo cryptobiosis and survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood mainly due to the absence of detailed knowledge about the proteome and genome of these organisms. Our study was intended to provide a basis for the functional characterization of expressed proteins in different states of tardigrades. High-throughput, high-accuracy proteomics in combination with a newly developed tardigrade specific protein database resulted in the identification of more than 3000 proteins in three different states: early embryonic state and adult animals in active and anhydrobiotic state. This comprehensive proteome resource includes protein families such as chaperones, antioxidants, ribosomal proteins, cytoskeletal proteins, transporters, protein channels, nutrient reservoirs, and developmental proteins. A comparative analysis of protein families in the different states was performed by calculating the exponentially modified protein abundance index which classifies proteins in major and minor components. This is the first step to analyzing the proteins involved in early embryonic development, and furthermore proteins which might play an important role in the transition into the anhydrobiotic state.…
Autor(en): | Elham Schokraie, Uwe Warnken, Agnes Hotz-Wagenblatt, Markus A. Grohme, Steffen Hengherr, Frank Förster, Ralph O. Schill, Marcus Frohme, Thomas Dandekar, Martina Schnölzer |
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URN: | urn:nbn:de:bvb:20-opus-134447 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS One |
Erscheinungsjahr: | 2012 |
Band / Jahrgang: | 7 |
Heft / Ausgabe: | 9 |
Seitenangabe: | e45682 |
Originalveröffentlichung / Quelle: | PLoS ONE 7(9): e45682. doi:10.1371/journal.pone.0045682 |
DOI: | https://doi.org/10.1371/journal.pone.0045682 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 0 Informatik, Informationswissenschaft, allgemeine Werke / 00 Informatik, Wissen, Systeme / 004 Datenverarbeitung; Informatik |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Freie Schlagwort(e): | Yolk protein; arabidopsis thaliana; caenorhabditis elegans; expression; genes; life-span regulation; richtersius coronifer; superoxide-dismutase; tolerance; vitellogenin; water stress |
Datum der Freischaltung: | 13.07.2017 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |