Low and High Field Magnetic Resonance for \(in\) \(Vivo\) Analysis of Seeds
Please always quote using this URN: urn:nbn:de:bvb:20-opus-140910
- Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can beLow field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.…
| Author: | Ljudmilla Borisjuk, Hardy Rolletschek, Johannes Fuchs, Gerd Melkus, Thomas Neuberger |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-140910 |
| Document Type: | Journal article |
| Faculties: | Fakultät für Physik und Astronomie / Physikalisches Institut |
| Language: | English |
| Parent Title (English): | Materials |
| Year of Completion: | 2011 |
| Volume: | 4 |
| Issue: | 8 |
| Pagenumber: | 1426-1439 |
| Source: | Materials 2011, 4, 1426-1439; doi:10.3390/ma4081426 |
| DOI: | https://doi.org/10.3390/ma4081426 |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 571 Physiologie und verwandte Themen |
| Tag: | (13)C; Crop seed; Deterioration; Gradients; H-1-NMR spectroscopy; Human brain; MRI; NMR; Oil; Plants; Soybean seeds; Storage; Time-domain NMR; Transport; lipid imaging; seed aging; seed quality; sucrose allocation |
| Release Date: | 2018/11/20 |
| Licence (German): |  CC BY: Creative-Commons-Lizenz: Namensnennung |