Tom70 Is Essential for PINK1 Import into Mitochondria

Please always quote using this URN: urn:nbn:de:bvb:20-opus-131061
  • PTEN induced kinase 1 (PINK1) is a serine/threonine kinase in the outer membrane of mitochondria (OMM), and known as a responsible gene of Parkinson's disease (PD). The precursor of PINK1 is synthesized in the cytosol and then imported into the mitochondria via the translocase of the OMM (TOM) complex. However, a large part of PINK1 import mechanism remains unclear. In this study, we examined using cell-free system the mechanism by which PINK1 is targeted to and assembled into mitochondria. Surprisingly, the main component of the importPTEN induced kinase 1 (PINK1) is a serine/threonine kinase in the outer membrane of mitochondria (OMM), and known as a responsible gene of Parkinson's disease (PD). The precursor of PINK1 is synthesized in the cytosol and then imported into the mitochondria via the translocase of the OMM (TOM) complex. However, a large part of PINK1 import mechanism remains unclear. In this study, we examined using cell-free system the mechanism by which PINK1 is targeted to and assembled into mitochondria. Surprisingly, the main component of the import channel, Tom40 was not necessary for PINK1 import. Furthermore, we revealed that the import receptor Tom70 is essential for PINK1 import. In addition, we observed that although PINK1 has predicted mitochondrial targeting signal, it was not processed by the mitochondrial processing peptidase. Thus, our results suggest that PINK1 is imported into mitochondria by a unique pathway that is independent of the TOM core complex but crucially depends on the import receptor Tom70.show moreshow less

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Metadaten
Author: Hiroki Kato, Qiping Lu, Doron Rapaport, Vera Kozjak-Pavlovic
URN:urn:nbn:de:bvb:20-opus-131061
Document Type:Journal article
Faculties:Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften
Language:English
Parent Title (English):PLoS ONE
Year of Completion:2013
Volume:8
Issue:3
Pagenumber:e58435
Source:PLoS ONE 8(3): e58435. doi:10.1371/ journal.pone.0058435
DOI:https://doi.org/10.1371/journal.pone.0058435
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 611 Menschliche Anatomie, Zytologie, Histologie
Tag:Parkinsons diesease; binding; biogenesis; complex; intracellular membranes; mechanisms; outer-membrane proteins; pathway; quality control; recruitment
Release Date:2016/05/12
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung